Restriction map for
pF1KE0652
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BanII BbeI BbvCI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsgI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 890 TCCGGA T^CCGGA
AcyI 388 GACGCC G[GA]^CG[CT]C
AhdI 607 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1138 GTGCAC G^TGCAC
ApaLI 1138 GTGCAC G^TGCAC
Asp700I 60 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 607 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1138 GTGCAC G[AT]GC[AT]^C
AvaI 5 CCCGGG C^[CT]CG[GA]G
BalI 1022 TGGCCA TGG^CCA
BbrPI 804 CACGTG CAC^GTG
BciVI 158 GTATCC GTATCC(6/5)
BclI 485 TGATCA T^GATCA
BglII 62 AGATCT A^GATCT
BmyI 1138 GTGCAC G[AGT]GC[ACT]^C
BpmI 551 CTCCAG CTGGAG(16/14)
BsaAI 804 CACGTG [CT]AC^GT[GA]
BsaHI 388 GACGCC G[GA]^CG[CT]C
BseAI 890 TCCGGA T^CCGGA
BsiEI 938 CGGCCG CG[GA][CT]^CG
BsiHKAI 1138 GTGCAC G[AT]GC[AT]^C
BsoBI 5 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1138 GTGCAC G[AGT]GC[ACT]^C
BspEI 890 TCCGGA T^CCGGA
BsrBI 1105 GAGCGG CCGCTC(-3/-3)
BssSI 868 CACGAG CACGAG(-5/-1)
BstAPI 925 GCATTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 938 CGGCCG C^GGCCG
Eam1105I 607 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 121 GAAGAG CTCTTC(1/4)
EclXI 938 CGGCCG C^GGCCG
Eco52I 938 CGGCCG C^GGCCG
EcoT14I 829 CCATGG C^C[AT][AT]GG
FbaI 485 TGATCA T^GATCA
HgaI 388 GACGC GACGC(5/10)
Hin1I 388 GACGCC G[GA]^CG[CT]C
HincII 604 GTTGAC GT[CT]^[GA]AC
HindII 604 GTTGAC GT[CT]^[GA]AC
Ksp632I 121 GAAGAG CTCTTC(1/4)
MaeII 805 ACGT A^CGT
MluNI 1022 TGGCCA TGG^CCA
MroI 890 TCCGGA T^CCGGA
MscI 1022 TGGCCA TGG^CCA
MspA1I 295 CAGCTG C[AC]G^C[GT]G
NcoI 829 CCATGG C^CATGG
NdeI 952 CATATG CA^TATG
PmaCI 804 CACGTG CAC^GTG
PmlI 804 CACGTG CAC^GTG
PpuMI 785 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 295 CAGCTG CAG^CTG
SmaI 5 CCCGGG CCC^GGG
SspI 238 AATATT AAT^ATT
StyI 829 CCATGG C^C[AT][AT]GG
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
XmnI 60 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 803 ACACGT A^C[GA][CT]GT
805 ACGTGT
AvaII 614 GGTCC G^G[AT]CC
786 GGACC
BanI 824 GGCACC G^G[CT][GA]CC
1044 GGCACC
BbsI 49 GAAGAC GAAGAC(2/6)
253 GAAGAC
BcgI 813 GCAGGCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
997 GCAAGTGTTTCG
BpuAI 49 GAAGAC GAAGAC(2/6)
253 GAAGAC
BseRI 27 CTCCTC GAGGAG(10/8)
469 GAGGAG
BsrI 133 ACTGG ACTGG(1/-1)
731 ACTGG
CfoI 172 GCGC GCG^C
881 GCGC
DraII 94 GGGGCCT [GA]G^G[ACGT]CC[CT]
785 AGGACCC
DsaI 829 CCATGG C^C[GA][CT]GG
935 CCACGG
EaeI 938 CGGCCG [CT]^GGCC[GA]
1022 TGGCCA
EcoO109I 94 GGGGCCT [GA]G^G[ACGT]CC[CT]
785 AGGACCC
HaeII 171 GGCGCT [GA]GCGC^[CT]
880 AGCGCC
HhaI 172 GCGC GCG^C
881 GCGC
HinP1I 172 GCGC G^CGC
881 GCGC
HphI 841 GGTGA GGTGA(8/7)
276 TCACC
MseI 227 TTAA T^TAA
929 TTAA
MslI 618 CATCGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1122 CACCACTGTG
PflMI 378 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
405 CCACAGAATGG
SfaNI 358 GATGC GCATC(5/9)
416 GCATC
SfcI 456 CTACAG C^T[GA][CT]AG
1059 CTGTAG
SmlI 433 CTCAAG C^T[CT][GA]AG
700 CTCAAG
TaqI 68 TCGA T^CGA
1006 TCGA
Tru9I 227 TTAA T^TAA
929 TTAA
TthHB8I 68 TCGA T^CGA
1006 TCGA
Van91I 378 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
405 CCACAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BsaI BsaJI BsaWI BsiYI
BslI BsmAI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI XhoII