Restriction map for
pF1KE0646
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BalI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MaeIII MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI TfiI Tsp45I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 673 AAATTC [GA]^AATT[CT]
AcyI 976 GGCGCC G[GA]^CG[CT]C
AfaI 507 GTAC GT^AC
Aor51HI 68 AGCGCT AGC^GCT
ApoI 673 AAATTC [GA]^AATT[CT]
AvaI 697 CCCGAG C^[CT]CG[GA]G
BamHI 894 GGATCC G^GATCC
BbeI 976 GGCGCC GGCGC^C
BbrPI 633 CACGTG CAC^GTG
BbsI 844 GAAGAC GAAGAC(2/6)
BlpI 420 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 420 GCTCAGC GC^T[ACGT]AGC
BpuAI 844 GAAGAC GAAGAC(2/6)
BsaAI 633 CACGTG [CT]AC^GT[GA]
BsaBI 603 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 976 GGCGCC G[GA]^CG[CT]C
BsaWI 414 TCCGGT [AT]^CCGG[AT]
BsiEI 923 CGGCCG CG[GA][CT]^CG
BsmFI 175 GTCCC GGGAC(10/14)
BsoBI 697 CCCGAG C^[CT]CG[GA]G
BspMI 495 ACCTGC ACCTGC(4/8)
BsrBI 1068 GAGCGG CCGCTC(-3/-3)
BssSI 533 CACGAG CACGAG(-5/-1)
BstAPI 771 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 894 GGATCC [GA]^GATC[CT]
CelII 420 GCTCAGC GC^T[ACGT]AGC
DraIII 1006 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 166 GACTCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 923 CGGCCG C^GGCCG
EclXI 923 CGGCCG C^GGCCG
Eco47III 68 AGCGCT AGC^GCT
Eco52I 923 CGGCCG C^GGCCG
EcoT14I 1022 CCAAGG C^C[AT][AT]GG
HgaI 1081 GACGC GACGC(5/10)
Hin1I 976 GGCGCC G[GA]^CG[CT]C
KasI 976 GGCGCC G^GCGCC
MaeII 634 ACGT A^CGT
MamI 603 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 894 GGATCC [GA]^GATC[CT]
MseI 137 TTAA T^TAA
MspA1I 1108 CAGCTG C[AC]G^C[GT]G
NarI 976 GGCGCC GG^CGCC
NspI 714 GCATGC [GA]CATG^[CT]
PmaCI 633 CACGTG CAC^GTG
PmlI 633 CACGTG CAC^GTG
PvuII 1108 CAGCTG CAG^CTG
RsaI 507 GTAC GT^AC
SacI 448 GAGCTC GAGCT^C
SfoI 976 GGCGCC GGC^GCC
SphI 714 GCATGC GCATG^C
StuI 1116 AGGCCT AGG^CCT
StyI 1022 CCAAGG C^C[AT][AT]GG
Tru9I 137 TTAA T^TAA
Tsp509I 674 AATT ^AATT
XcmI 107 CCAGCTCCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 894 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
###################################
BanI 976 GGCGCC G^G[CT][GA]CC
1134 GGCACC
DraII 281 AGGCCCT [GA]G^G[ACGT]CC[CT]
513 AGGGCCT
DsaI 116 CCGTGG C^C[GA][CT]GG
707 CCGTGG
EcoO109I 281 AGGCCCT [GA]G^G[ACGT]CC[CT]
513 AGGGCCT
HphI 120 GGTGA GGTGA(8/7)
276 GGTGA
MslI 678 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
871 CACCTCAATG
PflMI 466 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
834 CCAGACACTGG
PstI 453 CTGCAG CTGCA^G
586 CTGCAG
Van91I 466 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
834 CCAGACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI