Restriction map for
pF1KE0641
##################################
# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BclI BcnI BfaI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI Sau96I SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 596 GACGCC G[GA]^CG[CT]C
AfaI 200 GTAC GT^AC
AflII 345 CTTAAG C^TTAAG
AspHI 27 GAGCAC G[AT]GC[AT]^C
BamHI 480 GGATCC G^GATCC
BbvI 274 GCAGC GCAGC(8/12)
BcgI 362 CGAAAAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 40 GTATCC GTATCC(6/5)
BfrI 345 CTTAAG C^TTAAG
BmyI 27 GAGCAC G[AGT]GC[ACT]^C
BsaHI 596 GACGCC G[GA]^CG[CT]C
BsgI 188 CTGCAC GTGCAG(16/14)
BsiHKAI 27 GAGCAC G[AT]GC[AT]^C
BsmFI 571 GTCCC GGGAC(10/14)
BsmI 514 GCATTC GAATGC(1/-1)
Bsp1286I 27 GAGCAC G[AGT]GC[ACT]^C
BspHI 122 TCATGA T^CATGA
BsrDI 252 CATTGC GCAATG(2/0)
BsrI 560 CCAGT ACTGG(1/-1)
BstAPI 247 GCAAGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 114 GGTTACC G^GT[ACGT]ACC
BstF5I 285 CATCC GGATG(2/0)
BstPI 114 GGTTACC G^GT[ACGT]ACC
BstYI 480 GGATCC [GA]^GATC[CT]
DpnII 481 GATC ^GATC
EcoNI 498 CCTCAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 114 GGTTACC G^GT[ACGT]ACC
EcoT14I 601 CCAAGG C^C[AT][AT]GG
Fnu4HI 274 GCAGC GC^[ACGT]GC
FokI 285 CATCC GGATG(9/13)
HaeIII 507 GGCC GG^CC
HgaI 596 GACGC GACGC(5/10)
Hin1I 596 GACGCC G[GA]^CG[CT]C
ItaI 274 GCAGC GC^[ACGT]GC
MaeII 381 ACGT A^CGT
MaeIII 115 GTTAC ^GT[ACGT]AC
MboI 481 GATC ^GATC
MflI 480 GGATCC [GA]^GATC[CT]
MseI 346 TTAA T^TAA
MspA1I 275 CAGCTG C[AC]G^C[GT]G
MwoI 247 GCAAGCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 481 GATC ^GATC
NlaIII 123 CATG CATG^
PvuII 275 CAGCTG CAG^CTG
RcaI 122 TCATGA T^CATGA
RsaI 200 GTAC GT^AC
SapI 269 GAAGAGC GCTCTTC(1/4)
Sau3AI 481 GATC ^GATC
SmlI 345 CTTAAG C^T[CT][GA]AG
StyI 601 CCAAGG C^C[AT][AT]GG
TfiI 589 GATTC G^A[AT]TC
Tru9I 346 TTAA T^TAA
TseI 274 GCAGC G^C[AT]GC
XhoII 480 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 276 AGCT AG^CT
423 AGCT
AlwI 481 GATCC GGATC(4/5)
480 GGATC
BsaJI 203 CCAGGG C^C[ACGT][ACGT]GG
601 CCAAGG
BsiYI 498 CCTCAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
559 CCCAGTTCAGG
BslI 498 CCTCAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
559 CCCAGTTCAGG
BssKI 140 CCAGG ^CC[ACGT]GG
203 CCAGG
BstNI 140 CCAGG CC^[AT]GG
203 CCAGG
Cac8I 247 GCAAGC GC[ACGT]^[ACGT]GC
522 GCAAGC
EarI 144 GAAGAG CTCTTC(1/4)
269 GAAGAG
EcoRII 140 CCAGG ^CC[AT]GG
203 CCAGG
Ksp632I 144 GAAGAG CTCTTC(1/4)
269 GAAGAG
MvaI 140 CCAGG CC^[AT]GG
203 CCAGG
NlaIV 15 GGCTCC GG[ACGT]^[ACGT]CC
480 GGATCC
PleI 184 GACTC GAGTC(4/5)
569 GAGTC
ScrFI 140 CCAGG CC^[ACGT]GG
203 CCAGG
Tsp509I 444 AATT ^AATT
531 AATT
TspRI 32 CAGTG CA[GC]TG[ACGT][ACGT]^
627 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
DdeI HinfI MboII MnlI