Restriction map for
pF1KE0639
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BalI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaWI BseAI BsiEI BsmBI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcyI 571 GACGCC G[GA]^CG[CT]C
AflIII 488 ACATGT A^C[GA][CT]GT
AhdI 583 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 7 GGGCCC GGGCC^C
AspEI 583 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 544 CCCGAG C^[CT]CG[GA]G
AviII 280 TGCGCA TGC^GCA
BbrPI 511 CACGTG CAC^GTG
BbsI 346 GTCTTC GAAGAC(2/6)
BcnI 10 CCCGG CC^[GC]GG
BfaI 365 CTAG C^TAG
BglI 29 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 346 GTCTTC GAAGAC(2/6)
BsaBI 186 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 571 GACGCC G[GA]^CG[CT]C
BsaI 616 GAGACC GGTCTC(1/5)
BseRI 175 CTCCTC GAGGAG(10/8)
BsgI 223 GTGCAG GTGCAG(16/14)
BsiWI 71 CGTACG C^GTACG
BsmI 203 GCATTC GAATGC(1/-1)
BsoBI 544 CCCGAG C^[CT]CG[GA]G
Bsp1407I 83 TGTACA T^GTACA
BspLU11I 488 ACATGT A^CATGT
BsrGI 83 TGTACA T^GTACA
BsrI 462 ACTGG ACTGG(1/-1)
BssSI 110 CTCGTG CACGAG(-5/-1)
BstAPI 375 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 217 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 583 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 280 TGCGCA TGC^GCA
HapII 11 CCGG C^CGG
HgaI 571 GACGC GACGC(5/10)
Hin1I 571 GACGCC G[GA]^CG[CT]C
HinfI 321 GATTC G^A[ACGT]TC
HpaII 11 CCGG C^CGG
MaeI 365 CTAG C^TAG
MaeIII 251 GTGAC ^GT[ACGT]AC
MamI 186 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 347 TCTTC GAAGA(8/7)
MspI 11 CCGG C^CGG
NciI 10 CCCGG CC^[GC]GG
NcoI 593 CCATGG C^CATGG
NheI 364 GCTAGC G^CTAGC
NspI 488 ACATGT [GA]CATG^[CT]
PmaCI 511 CACGTG CAC^GTG
PmlI 511 CACGTG CAC^GTG
PpuMI 18 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 226 CAGCTG CAG^CTG
SacI 435 GAGCTC GAGCT^C
SfaNI 160 GCATC GCATC(5/9)
SmlI 196 CTCAAG C^T[CT][GA]AG
SspBI 83 TGTACA T^GTACA
StuI 89 AGGCCT AGG^CCT
TaqI 207 TCGA T^CGA
TfiI 321 GATTC G^A[AT]TC
Tsp45I 251 GTGAC ^GT[GC]AC
TthHB8I 207 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 72 GTAC GT^AC
84 GTAC
AlwI 192 GATCC GGATC(4/5)
408 GGATC
AspHI 435 GAGCTC G[AT]GC[AT]^C
514 GTGCTC
BanII 7 GGGCCC G[GA]GC[CT]^C
435 GAGCTC
BsaAI 69 CACGTA [CT]AC^GT[GA]
511 CACGTG
BsiHKAI 435 GAGCTC G[AT]GC[AT]^C
514 GTGCTC
BsmAI 616 GAGAC GTCTC(1/5)
297 GTCTC
BstYI 191 AGATCC [GA]^GATC[CT]
408 GGATCT
DdeI 329 CTGAG C^T[ACGT]AG
433 CTGAG
DpnII 192 GATC ^GATC
409 GATC
DraII 6 GGGGCCC [GA]G^G[ACGT]CC[CT]
18 GGGTCCC
EcoO109I 6 GGGGCCC [GA]G^G[ACGT]CC[CT]
18 GGGTCCC
EcoT14I 440 CCTTGG C^C[AT][AT]GG
593 CCATGG
HaeII 53 AGCGCC [GA]GCGC^[CT]
367 AGCGCC
MaeII 70 ACGT A^CGT
512 ACGT
MboI 192 GATC ^GATC
409 GATC
MflI 191 AGATCC [GA]^GATC[CT]
408 GGATCT
NdeII 192 GATC ^GATC
409 GATC
RsaI 72 GTAC GT^AC
84 GTAC
Sau3AI 192 GATC ^GATC
409 GATC
StyI 440 CCTTGG C^C[AT][AT]GG
593 CCATGG
Tsp509I 359 AATT ^AATT
377 AATT
TspRI 241 CAGTG CA[GC]TG[ACGT][ACGT]^
249 CAGTG
XhoII 191 AGATCC [GA]^GATC[CT]
408 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DsaI EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MnlI MspA1I
MvaI MvnI MwoI NlaIII NlaIV
Sau96I ScrFI TseI