Restriction map for
pF1KE0637
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsiWI BsmAI BsmBI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI HincII HindII
HindIII HpaI KspI MaeIII MamI
MfeI MflI MluI MunI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI TaqI
TfiI Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 575 GGTACC G^GTACC
AccII 57 CGCG CG^CG
AccIII 89 TCCGGA T^CCGGA
AcyI 70 GGCGCC G[GA]^CG[CT]C
Alw44I 376 GTGCAC G^TGCAC
AlwNI 295 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 464 GGGCCC GGGCC^C
ApaLI 376 GTGCAC G^TGCAC
Asp718I 575 GGTACC G^GTACC
AvrII 387 CCTAGG C^CTAGG
BalI 335 TGGCCA TGG^CCA
BbeI 70 GGCGCC GGCGC^C
BclI 182 TGATCA T^GATCA
BlnI 387 CCTAGG C^CTAGG
BpmI 76 CTGGAG CTGGAG(16/14)
BsaHI 70 GGCGCC G[GA]^CG[CT]C
BsaWI 89 TCCGGA [AT]^CCGG[AT]
BseAI 89 TCCGGA T^CCGGA
BsgI 233 GTGCAG GTGCAG(16/14)
BsiEI 154 CGGCCG CG[GA][CT]^CG
BsmI 255 GAATGC GAATGC(1/-1)
Bsp1407I 479 TGTACA T^GTACA
BspEI 89 TCCGGA T^CCGGA
BsrGI 479 TGTACA T^GTACA
BstUI 57 CGCG CG^CG
BstXI 454 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 154 CGGCCG C^GGCCG
EarI 148 CTCTTC CTCTTC(1/4)
EclXI 154 CGGCCG C^GGCCG
Eco52I 154 CGGCCG C^GGCCG
EcoNI 75 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 387 CCTAGG C^C[AT][AT]GG
FbaI 182 TGATCA T^GATCA
HaeII 70 GGCGCC [GA]GCGC^[CT]
Hin1I 70 GGCGCC G[GA]^CG[CT]C
HinfI 297 GACTC G^A[ACGT]TC
HphI 521 TCACC GGTGA(8/7)
KasI 70 GGCGCC G^GCGCC
KpnI 575 GGTACC GGTAC^C
Ksp632I 148 CTCTTC CTCTTC(1/4)
MaeII 374 ACGT A^CGT
MboII 149 TCTTC GAAGA(8/7)
MluNI 335 TGGCCA TGG^CCA
MroI 89 TCCGGA T^CCGGA
MscI 335 TGGCCA TGG^CCA
MseI 125 TTAA T^TAA
MslI 327 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 57 CGCG CG^CG
NarI 70 GGCGCC GG^CGCC
NlaIII 511 CATG CATG^
PleI 297 GACTC GAGTC(4/5)
SapI 147 GCTCTTC GCTCTTC(1/4)
SfaNI 448 GCATC GCATC(5/9)
SfoI 70 GGCGCC GGC^GCC
SspBI 479 TGTACA T^GTACA
StuI 458 AGGCCT AGG^CCT
StyI 387 CCTAGG C^C[AT][AT]GG
Tru9I 125 TTAA T^TAA
XcmI 323 CCAACACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 280 GATCC GGATC(4/5)
441 GATCC
BanII 60 GGGCTC G[GA]GC[CT]^C
464 GGGCCC
BfaI 307 CTAG C^TAG
388 CTAG
BseRI 79 GAGGAG GAGGAG(10/8)
82 GAGGAG
BsmFI 410 GGGAC GGGAC(10/14)
177 GTCCC
BsrFI 40 ACCGGC [GA]^CCGG[CT]
46 ACCGGC
BssKI 75 CCTGG ^CC[ACGT]GG
461 CCTGG
BstF5I 449 CATCC GGATG(2/0)
604 GGATG
BstNI 75 CCTGG CC^[AT]GG
461 CCTGG
Cfr10I 40 ACCGGC [GA]^CCGG[CT]
46 ACCGGC
DdeI 301 CTGAG C^T[ACGT]AG
563 CTCAG
EcoRII 75 CCTGG ^CC[AT]GG
461 CCTGG
FauI 382 CCCGC CCCGC(4/6)
58 GCGGG
FokI 449 CATCC GGATG(9/13)
604 GGATG
MaeI 307 CTAG C^TAG
388 CTAG
MspA1I 105 CAGCGG C[AC]G^C[GT]G
542 CCGCTG
MvaI 75 CCTGG CC^[AT]GG
461 CCTGG
PpuMI 175 GGGTCCC [GA]G^G[AT]CC[CT]
410 GGGACCC
ScrFI 75 CCTGG CC^[ACGT]GG
461 CCTGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BbvI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I Cac8I CfoI
Cfr13I DpnII DraII EaeI EcoO109I
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MnlI MspI
MwoI NdeII NlaIV RsaI Sau3AI
Sau96I TseI TspRI