Restriction map for
pF1KE0633
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AhdI
AlwI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV FseI
FspI HaeII HgaI Hin1I HindIII
HinfI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 233 TCCGGA T^CCGGA
AfaI 313 GTAC GT^AC
AgeI 92 ACCGGT A^CCGGT
Alw44I 301 GTGCAC G^TGCAC
ApaLI 301 GTGCAC G^TGCAC
BalI 425 TGGCCA TGG^CCA
BanII 594 GGGCTC G[GA]GC[CT]^C
BbvI 374 GCAGC GCAGC(8/12)
BclI 464 TGATCA T^GATCA
BpmI 366 CTCCAG CTGGAG(16/14)
BseAI 233 TCCGGA T^CCGGA
BspEI 233 TCCGGA T^CCGGA
BspMI 400 GCAGGT ACCTGC(4/8)
BsrBI 253 GAGCGG CCGCTC(-3/-3)
BssSI 208 CTCGTG CACGAG(-5/-1)
BstAPI 294 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 403 GGTCACC G^GT[ACGT]ACC
BstPI 403 GGTCACC G^GT[ACGT]ACC
CfoI 398 GCGC GCG^C
DraIII 205 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 260 CCGTGG C^C[GA][CT]GG
EarI 172 GAAGAG CTCTTC(1/4)
EcoO65I 403 GGTCACC G^GT[ACGT]ACC
EcoT14I 195 CCTTGG C^C[AT][AT]GG
EcoT22I 339 ATGCAT ATGCA^T
FbaI 464 TGATCA T^GATCA
HhaI 398 GCGC GCG^C
HinP1I 398 GCGC G^CGC
HincII 46 GTTAAC GT[CT]^[GA]AC
HindII 46 GTTAAC GT[CT]^[GA]AC
HpaI 46 GTTAAC GTT^AAC
Ksp632I 172 GAAGAG CTCTTC(1/4)
MaeII 218 ACGT A^CGT
MboII 172 GAAGA GAAGA(8/7)
MluNI 425 TGGCCA TGG^CCA
MroI 233 TCCGGA T^CCGGA
MscI 425 TGGCCA TGG^CCA
MspA1I 279 CCGCTG C[AC]G^C[GT]G
NaeI 80 GCCGGC GCC^GGC
NgoMIV 80 GCCGGC G^CCGGC
NsiI 339 ATGCAT ATGCA^T
PflMI 204 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 92 ACCGGT A^CCGGT
PstI 148 CTGCAG CTGCA^G
RsaI 313 GTAC GT^AC
SfaNI 341 GCATC GCATC(5/9)
SfcI 148 CTGCAG C^T[GA][CT]AG
SmaI 591 CCCGGG CCC^GGG
SspI 8 AATATT AAT^ATT
StyI 195 CCTTGG C^C[AT][AT]GG
TaqI 19 TCGA T^CGA
TseI 374 GCAGC G^C[AT]GC
Tsp45I 404 GTCAC ^GT[GC]AC
TspRI 468 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 19 TCGA T^CGA
Van91I 204 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 591 CCCGGG C^CCGGG
XmaI 591 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 15 AGCT AG^CT
376 AGCT
AspHI 96 GTGCTC G[AT]GC[AT]^C
301 GTGCAC
BsaWI 92 ACCGGT [AT]^CCGG[AT]
233 TCCGGA
BseRI 546 CTCCTC GAGGAG(10/8)
241 GAGGAG
BsiHKAI 96 GTGCTC G[AT]GC[AT]^C
301 GTGCAC
BsrFI 80 GCCGGC [GA]^CCGG[CT]
92 ACCGGT
BsrI 423 ACTGG ACTGG(1/-1)
469 ACTGG
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
92 ACCGGT
DpnII 69 GATC ^GATC
465 GATC
EaeI 425 TGGCCA [CT]^GGCC[GA]
476 TGGCCG
FauI 255 GCGGG CCCGC(4/6)
386 GCGGG
HphI 405 TCACC GGTGA(8/7)
509 TCACC
MaeIII 275 GTAAC ^GT[ACGT]AC
404 GTCAC
MboI 69 GATC ^GATC
465 GATC
MseI 47 TTAA T^TAA
65 TTAA
MslI 304 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
468 CACTGGCATG
NdeII 69 GATC ^GATC
465 GATC
NlaIII 338 CATG CATG^
474 CATG
PpuMI 389 GGGACCC [GA]G^G[AT]CC[CT]
539 AGGACCT
Sau3AI 69 GATC ^GATC
465 GATC
Tru9I 47 TTAA T^TAA
65 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AvaI AvaII BcnI BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI