Restriction map for
pF1KE0631
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI AlwI AlwNI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BbvI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I CelII CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MflI MluNI MroI
MscI MseI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspI
StuI SwaI Tru9I TseI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AatII 553 GACGTC GACGT^C
AccII 135 CGCG CG^CG
AcsI 473 AAATTT [GA]^AATT[CT]
AflIII 134 ACGCGT A^C[GA][CT]GT
AgeI 156 ACCGGT A^CCGGT
AluI 72 AGCT AG^CT
Alw44I 400 GTGCAC G^TGCAC
AocI 31 CCTGAGG CC^T[ACGT]AGG
ApaLI 400 GTGCAC G^TGCAC
ApoI 473 AAATTT [GA]^AATT[CT]
AspHI 400 GTGCAC G[AT]GC[AT]^C
AviII 218 TGCGCA TGC^GCA
BfaI 545 CTAG C^TAG
BglI 166 GCCAAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 178 CTGGAG CTGGAG(16/14)
BsaAI 13 TACGTG [CT]AC^GT[GA]
BsaI 181 GAGACC GGTCTC(1/5)
BsaWI 156 ACCGGT [AT]^CCGG[AT]
BseRI 583 GAGGAG GAGGAG(10/8)
BsgI 25 CTGCAC GTGCAG(16/14)
BsiHKAI 400 GTGCAC G[AT]GC[AT]^C
BsmAI 181 GAGAC GTCTC(1/5)
Bsp1407I 317 TGTACA T^GTACA
BspDI 436 ATCGAT AT^CGAT
BspMI 23 ACCTGC ACCTGC(4/8)
BsrDI 46 GCAATG GCAATG(2/0)
BsrFI 156 ACCGGT [GA]^CCGG[CT]
BsrGI 317 TGTACA T^GTACA
BsrI 214 CCAGT ACTGG(1/-1)
BstEII 36 GGTGACC G^GT[ACGT]ACC
BstPI 36 GGTGACC G^GT[ACGT]ACC
BstUI 135 CGCG CG^CG
Bsu36I 31 CCTGAGG CC^T[ACGT]AGG
Cfr10I 156 ACCGGT [GA]^CCGG[CT]
ClaI 436 ATCGAT AT^CGAT
DdeI 32 CTGAG C^T[ACGT]AG
DpnII 111 GATC ^GATC
Eco81I 31 CCTGAGG CC^T[ACGT]AGG
EcoNI 527 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 36 GGTGACC G^GT[ACGT]ACC
EcoT14I 383 CCAAGG C^C[AT][AT]GG
Fnu4HI 119 GCCGC GC^[ACGT]GC
FspI 218 TGCGCA TGC^GCA
HaeII 312 GGCGCT [GA]GCGC^[CT]
HapII 157 CCGG C^CGG
HgaI 163 GACGC GACGC(5/10)
HincII 142 GTCAAC GT[CT]^[GA]AC
HindII 142 GTCAAC GT[CT]^[GA]AC
HpaII 157 CCGG C^CGG
ItaI 119 GCCGC GC^[ACGT]GC
MaeI 545 CTAG C^TAG
MboI 111 GATC ^GATC
MluI 134 ACGCGT A^CGCGT
MslI 435 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 71 CAGCTG C[AC]G^C[GT]G
MspI 157 CCGG C^CGG
MvnI 135 CGCG CG^CG
NdeII 111 GATC ^GATC
NspI 452 GCATGT [GA]CATG^[CT]
PinAI 156 ACCGGT A^CCGGT
PpuMI 239 GGGACCC [GA]G^G[AT]CC[CT]
PstI 99 CTGCAG CTGCA^G
PvuII 71 CAGCTG CAG^CTG
Sau3AI 111 GATC ^GATC
SfcI 99 CTGCAG C^T[GA][CT]AG
SpeI 544 ACTAGT A^CTAGT
SspBI 317 TGTACA T^GTACA
StyI 383 CCAAGG C^C[AT][AT]GG
Tsp509I 474 AATT ^AATT
TspRI 215 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 167 CCAAGCGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 163 GACGCC G[GA]^CG[CT]C
553 GACGTC
AvaII 230 GGTCC G^G[AT]CC
240 GGACC
BbsI 571 GAAGAC GAAGAC(2/6)
274 GTCTTC
BcgI 293 GCAAGACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
470 GCAAAATTTTCG
BpuAI 571 GAAGAC GAAGAC(2/6)
274 GTCTTC
BsaHI 163 GACGCC G[GA]^CG[CT]C
553 GACGTC
CfoI 219 GCGC GCG^C
313 GCGC
HhaI 219 GCGC GCG^C
313 GCGC
Hin1I 163 GACGCC G[GA]^CG[CT]C
553 GACGTC
HinP1I 219 GCGC G^CGC
313 GCGC
HinfI 418 GAATC G^A[ACGT]TC
488 GATTC
HphI 36 GGTGA GGTGA(8/7)
113 TCACC
MaeIII 37 GTGAC ^GT[ACGT]AC
368 GTGAC
NlaIII 225 CATG CATG^
453 CATG
SfaNI 95 GCATC GCATC(5/9)
221 GCATC
TfiI 418 GAATC G^A[AT]TC
488 GATTC
Tsp45I 37 GTGAC ^GT[GC]AC
368 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI ApaI BanII BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrBI BssKI BstF5I BstNI Cac8I
Cfr13I DraII EcoO109I EcoRII FauI
FokI HaeIII MaeII MboII MnlI
MvaI MwoI NlaIV RsaI Sau96I
ScrFI TaqI TthHB8I