Restriction map for
pF1KE0620
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BpmI BsaAI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsmI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HindIII HpaI KpnI
KspI MaeI MfeI MflI MluI
MroI MseI MunI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SbfI
ScaI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AhdI 190 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 265 GATCC GGATC(4/5)
ApaI 45 GGGCCC GGGCC^C
AspEI 190 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 475 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 494 CTCGGG C^[CT]CG[GA]G
AvaII 474 GGACC G^G[AT]CC
BalI 998 TGGCCA TGG^CCA
BanI 820 GGCGCC G^G[CT][GA]CC
BbeI 820 GGCGCC GGCGC^C
BcgI 950 GCAAGGGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 583 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 583 GCTGAGC GC^T[ACGT]AGC
BsaBI 762 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1047 GGTCTC GGTCTC(1/5)
BsaWI 855 ACCGGA [AT]^CCGG[AT]
BsoBI 494 CTCGGG C^[CT]CG[GA]G
Bsp1407I 842 TGTACA T^GTACA
BspDI 769 ATCGAT AT^CGAT
BsrBI 780 GAGCGG CCGCTC(-3/-3)
BsrGI 842 TGTACA T^GTACA
BstAPI 633 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 583 GCTGAGC GC^T[ACGT]AGC
ClaI 769 ATCGAT AT^CGAT
Eam1105I 190 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 51 GGCCGGCC GGCCGG^CC
HgaI 743 GCGTC GACGC(5/10)
HincII 127 GTCAAC GT[CT]^[GA]AC
HindII 127 GTCAAC GT[CT]^[GA]AC
KasI 820 GGCGCC G^GCGCC
MamI 762 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 998 TGGCCA TGG^CCA
MscI 998 TGGCCA TGG^CCA
NarI 820 GGCGCC GG^CGCC
NruI 605 TCGCGA TCG^CGA
PflFI 475 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 76 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1042 CTGCAG CTGCA^G
PvuI 761 CGATCG CGAT^CG
SapI 429 GCTCTTC GCTCTTC(1/4)
SexAI 476 ACCAGGT A^CC[AT]GGT
SfcI 1042 CTGCAG C^T[GA][CT]AG
SfoI 820 GGCGCC GGC^GCC
SspBI 842 TGTACA T^GTACA
TfiI 506 GATTC G^A[AT]TC
TspRI 99 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 475 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 76 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 742 GGCGTC G[GA]^CG[CT]C
820 GGCGCC
AlwNI 10 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
634 CAGGCGCTG
BbsI 321 GAAGAC GAAGAC(2/6)
624 GAAGAC
BpuAI 321 GAAGAC GAAGAC(2/6)
624 GAAGAC
BsaHI 742 GGCGTC G[GA]^CG[CT]C
820 GGCGCC
BsmFI 215 GGGAC GGGAC(10/14)
459 GTCCC
BspMI 134 ACCTGC ACCTGC(4/8)
1044 GCAGGT
BsrFI 52 GCCGGC [GA]^CCGG[CT]
823 GCCGGC
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
823 GCCGGC
DraII 44 GGGGCCC [GA]G^G[ACGT]CC[CT]
681 AGGCCCC
DsaI 23 CCATGG C^C[GA][CT]GG
995 CCATGG
EagI 50 CGGCCG C^GGCCG
444 CGGCCG
EclXI 50 CGGCCG C^GGCCG
444 CGGCCG
Eco52I 50 CGGCCG C^GGCCG
444 CGGCCG
EcoO109I 44 GGGGCCC [GA]G^G[ACGT]CC[CT]
681 AGGCCCC
Hin1I 742 GGCGTC G[GA]^CG[CT]C
820 GGCGCC
NaeI 52 GCCGGC GCC^GGC
823 GCCGGC
NcoI 23 CCATGG C^CATGG
995 CCATGG
NgoMIV 52 GCCGGC G^CCGGC
823 GCCGGC
PvuII 581 CAGCTG CAG^CTG
883 CAGCTG
SfiI 46 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
51 GGCCGGCCCGGCC
TaqI 194 TCGA T^CGA
770 TCGA
Tsp509I 311 AATT ^AATT
721 AATT
TthHB8I 194 TCGA T^CGA
770 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI BanII
BbvI BcnI BglI BmyI BsaJI
BsiEI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I