Restriction map for
pF1KE0616
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
Asp700I AspEI AvaII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI HgaI Hin1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MflI MluI MluNI MroI
MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TspRI
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 346 GGTACC G^GTACC
AcsI 331 GAATTC [GA]^AATT[CT]
AflIII 551 ACGTGT A^C[GA][CT]GT
AlwI 15 GATCC GGATC(4/5)
Aor51HI 418 AGCGCT AGC^GCT
ApoI 331 GAATTC [GA]^AATT[CT]
Asp718I 346 GGTACC G^GTACC
AviII 192 TGCGCA TGC^GCA
BciVI 936 GTATCC GTATCC(6/5)
BsmFI 166 GGGAC GGGAC(10/14)
BsrBI 730 CCGCTC CCGCTC(-3/-3)
BsrFI 791 ACCGGC [GA]^CCGG[CT]
BsrI 600 CCAGT ACTGG(1/-1)
Cac8I 895 GCCCGC GC[ACGT]^[ACGT]GC
Cfr10I 791 ACCGGC [GA]^CCGG[CT]
DpnII 15 GATC ^GATC
DsaI 699 CCATGG C^C[GA][CT]GG
Eco47III 418 AGCGCT AGC^GCT
EcoRI 331 GAATTC G^AATTC
EcoT14I 699 CCATGG C^C[AT][AT]GG
FspI 192 TGCGCA TGC^GCA
HaeII 418 AGCGCT [GA]GCGC^[CT]
KpnI 346 GGTACC GGTAC^C
MaeII 551 ACGT A^CGT
MboI 15 GATC ^GATC
MfeI 437 CAATTG C^AATTG
MseI 206 TTAA T^TAA
MslI 184 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 437 CAATTG C^AATTG
NcoI 699 CCATGG C^CATGG
NdeII 15 GATC ^GATC
PshAI 690 GACGAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 680 GAGCTC GAGCT^C
Sau3AI 15 GATC ^GATC
SmaI 21 CCCGGG CCC^GGG
SmlI 835 CTCAAG C^T[CT][GA]AG
StyI 699 CCATGG C^C[AT][AT]GG
TfiI 316 GAATC G^A[AT]TC
Tru9I 206 TTAA T^TAA
XmaCI 21 CCCGGG C^CCGGG
XmaI 21 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 24 GGGCCC GGGCC^C
365 GGGCCC
AspHI 619 GTGCTC G[AT]GC[AT]^C
680 GAGCTC
AspI 168 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
922 GACACGGTC
AvaI 21 CCCGGG C^[CT]CG[GA]G
541 CCCGAG
BcnI 21 CCCGG CC^[GC]GG
22 CCGGG
BfaI 455 CTAG C^TAG
503 CTAG
BglI 435 GCCAATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
599 GCCAGTCGGGC
BpmI 622 CTCCAG CTGGAG(16/14)
134 CTGGAG
BsaI 263 GGTCTC GGTCTC(1/5)
493 GGTCTC
BsaWI 557 ACCGGA [AT]^CCGG[AT]
684 TCCGGT
BsiHKAI 619 GTGCTC G[AT]GC[AT]^C
680 GAGCTC
BsoBI 21 CCCGGG C^[CT]CG[GA]G
541 CCCGAG
DraII 24 GGGCCCT [GA]G^G[ACGT]CC[CT]
364 AGGGCCC
EcoO109I 24 GGGCCCT [GA]G^G[ACGT]CC[CT]
364 AGGGCCC
HaeIII 25 GGCC GG^CC
366 GGCC
MaeI 455 CTAG C^TAG
503 CTAG
MboII 280 GAAGA GAAGA(8/7)
854 TCTTC
NciI 21 CCCGG CC^[GC]GG
22 CCGGG
PflFI 168 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
922 GACACGGTC
PflMI 72 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
624 CCAGGGAATGG
Tth111I 168 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
922 GACACGGTC
Van91I 72 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
624 CCAGGGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstUI CfoI Cfr13I DdeI
EcoRII FauI Fnu4HI FokI HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MnlI MspA1I MspI
MvaI MvnI MwoI NlaIII NlaIV
PleI PvuII RsaI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I