Restriction map for
pF1KE0610
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MflI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 132 GCGG CCGC(-3/-1)
AclI 1024 AACGTT AA^CGTT
AcsI 225 AAATTC [GA]^AATT[CT]
AlwNI 506 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 225 AAATTC [GA]^AATT[CT]
AseI 981 ATTAAT AT^TAAT
AsnI 981 ATTAAT AT^TAAT
Asp700I 1023 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 348 TGGCCA TGG^CCA
BbvI 815 GCAGC GCAGC(8/12)
BclI 219 TGATCA T^GATCA
BsgI 274 GTGCAG GTGCAG(16/14)
BsmFI 857 GGGAC GGGAC(10/14)
BspMI 866 ACCTGC ACCTGC(4/8)
BsrDI 684 CATTGC GCAATG(2/0)
BstAPI 267 GCAATCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 732 CCAGGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 220 GATC ^GATC
DraI 286 TTTAAA TTT^AAA
DraII 857 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 348 TGGCCA [CT]^GGCC[GA]
EcoNI 144 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 857 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 486 CCTTGG C^C[AT][AT]GG
FbaI 219 TGATCA T^GATCA
Fnu4HI 815 GCAGC GC^[ACGT]GC
ItaI 815 GCAGC GC^[ACGT]GC
MaeII 1025 ACGT A^CGT
MaeIII 115 GTGAC ^GT[ACGT]AC
MboI 220 GATC ^GATC
MfeI 970 CAATTG C^AATTG
MluNI 348 TGGCCA TGG^CCA
MscI 348 TGGCCA TGG^CCA
MslI 295 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 100 CAGCTG C[AC]G^C[GT]G
MunI 970 CAATTG C^AATTG
NdeII 220 GATC ^GATC
PflMI 529 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 857 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 981 ATTAAT AT^TAAT
Psp1406I 1024 AACGTT AA^CGTT
PvuII 100 CAGCTG CAG^CTG
Sau3AI 220 GATC ^GATC
SexAI 731 ACCAGGT A^CC[AT]GGT
SfaNI 264 GATGC GCATC(5/9)
SmlI 617 CTCAAG C^T[CT][GA]AG
StyI 486 CCTTGG C^C[AT][AT]GG
TaqI 654 TCGA T^CGA
TseI 815 GCAGC G^C[AT]GC
Tsp45I 115 GTGAC ^GT[GC]AC
TthHB8I 654 TCGA T^CGA
Van91I 529 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1023 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 6 enzymes) #
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AspI 373 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
730 GACCAGGTC
AvaII 724 GGTCC G^G[AT]CC
858 GGACC
PflFI 373 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
730 GACCAGGTC
PleI 21 GAGTC GAGTC(4/5)
802 GAGTC
TspRI 231 CAGTG CA[GC]TG[ACGT][ACGT]^
693 CAGTG
Tth111I 373 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
730 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cfr13I DdeI EarI
EcoRII FokI HaeIII HinfI HphI
Ksp632I MboII MnlI MseI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TfiI Tru9I Tsp509I