Restriction map for
pF1KE0603
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BmyI BpmI
BpuAI BsaHI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MflI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 310 ACGCGT A^C[GA][CT]GT
Asp700I 134 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 63 GGACC G^G[AT]CC
AviII 26 TGCGCA TGC^GCA
BlpI 48 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
BsaAI 40 TACGTG [CT]AC^GT[GA]
BsaBI 406 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 437 GAGGAG GAGGAG(10/8)
BsiEI 375 CGGCCG CG[GA][CT]^CG
BstNI 396 CCAGG CC^[AT]GG
CelII 48 GCTGAGC GC^T[ACGT]AGC
DdeI 49 CTGAG C^T[ACGT]AG
DpnII 386 GATC ^GATC
DsaI 378 CCGTGG C^C[GA][CT]GG
EaeI 375 CGGCCG [CT]^GGCC[GA]
EagI 375 CGGCCG C^GGCCG
EarI 175 CTCTTC CTCTTC(1/4)
EclXI 375 CGGCCG C^GGCCG
Eco52I 375 CGGCCG C^GGCCG
EcoNI 12 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 396 CCAGG ^CC[AT]GG
FspI 26 TGCGCA TGC^GCA
HaeII 21 GGCGCT [GA]GCGC^[CT]
Ksp632I 175 CTCTTC CTCTTC(1/4)
MamI 406 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 386 GATC ^GATC
MluI 310 ACGCGT A^CGCGT
MseI 356 TTAA T^TAA
MvaI 396 CCAGG CC^[AT]GG
MwoI 280 GCGGAAAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 386 GATC ^GATC
NspI 238 GCATGT [GA]CATG^[CT]
PleI 34 GACTC GAGTC(4/5)
Sau3AI 386 GATC ^GATC
SspI 260 AATATT AAT^ATT
Tru9I 356 TTAA T^TAA
Tsp509I 101 AATT ^AATT
XmnI 134 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 271 CGCG CG^CG
311 CGCG
AciI 280 GCGG CCGC(-3/-1)
294 GCGG
AluI 47 AGCT AG^CT
115 AGCT
BbvI 29 GCAGC GCAGC(8/12)
24 GCTGC
BcnI 60 CCGGG CC^[GC]GG
75 CCGGG
BsaJI 75 CCGGGG C^C[ACGT][ACGT]GG
378 CCGTGG
BstF5I 318 GGATG GGATG(2/0)
405 GGATG
BstUI 271 CGCG CG^CG
311 CGCG
Cac8I 349 GCAGGC GC[ACGT]^[ACGT]GC
365 GCAGGC
Cfr13I 63 GGACC G^G[ACGT]CC
393 GGGCC
Fnu4HI 24 GCTGC GC^[ACGT]GC
29 GCAGC
FokI 318 GGATG GGATG(9/13)
405 GGATG
HapII 60 CCGG C^CGG
75 CCGG
HpaII 60 CCGG C^CGG
75 CCGG
HphI 79 GGTGA GGTGA(8/7)
190 GGTGA
ItaI 24 GCTGC GC^[ACGT]GC
29 GCAGC
MaeII 41 ACGT A^CGT
121 ACGT
MaeIII 80 GTGAC ^GT[ACGT]AC
191 GTGAC
MspI 60 CCGG C^CGG
75 CCGG
MvnI 271 CGCG CG^CG
311 CGCG
NciI 60 CCGGG CC^[GC]GG
75 CCGGG
NlaIII 239 CATG CATG^
291 CATG
NlaIV 62 GGGACC GG[ACGT]^[ACGT]CC
392 GGGGCC
Sau96I 63 GGACC G^G[ACGT]CC
393 GGGCC
SfaNI 277 GATGC GCATC(5/9)
427 GATGC
TfiI 245 GATTC G^A[AT]TC
326 GAATC
TseI 24 GCTGC G^C[AT]GC
29 GCAGC
Tsp45I 80 GTGAC ^GT[GC]AC
191 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AfaI BsiYI BslI BsmFI BsrI
BssKI CfoI HaeIII HgaI HhaI
HinP1I HinfI MboII MnlI RsaI
ScrFI