Restriction map for
pF1KE0589
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspEI
AvrII BbrPI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoT22I FbaI
FseI HindIII HpaI KpnI Ksp632I
KspI MaeI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I TthHB8I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwNI 247 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 543 GGCGCGCC GG^CGCGCC
AspHI 1062 GAGCAC G[AT]GC[AT]^C
AspI 928 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 692 TGCGCA TGC^GCA
BamHI 261 GGATCC G^GATCC
BanII 944 GGGCTC G[GA]GC[CT]^C
BbeI 1110 GGCGCC GGCGC^C
BbvCI 165 GCTGAGG CCTCAGC(-5/-2)
BlpI 405 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 405 GCTGAGC GC^T[ACGT]AGC
BsaAI 1144 TACGTG [CT]AC^GT[GA]
BsaBI 741 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 170 GGTCTC GGTCTC(1/5)
BseRI 1023 GAGGAG GAGGAG(10/8)
BsiHKAI 1062 GAGCAC G[AT]GC[AT]^C
BsmI 138 GCATTC GAATGC(1/-1)
BspMI 1298 ACCTGC ACCTGC(4/8)
BsrDI 834 CATTGC GCAATG(2/0)
BssHII 544 GCGCGC G^CGCGC
BssSI 368 CACGAG CACGAG(-5/-1)
BstEII 990 GGTCACC G^GT[ACGT]ACC
BstPI 990 GGTCACC G^GT[ACGT]ACC
CelII 405 GCTGAGC GC^T[ACGT]AGC
DrdI 1165 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 20 CCATGG C^C[GA][CT]GG
EcoNI 645 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 990 GGTCACC G^GT[ACGT]ACC
EcoRV 607 GATATC GAT^ATC
FspI 692 TGCGCA TGC^GCA
HgaI 467 GCGTC GACGC(5/10)
KasI 1110 GGCGCC G^GCGCC
MamI 741 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1110 GGCGCC GG^CGCC
NcoI 20 CCATGG C^CATGG
PflFI 928 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 681 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfoI 1110 GGCGCC GGC^GCC
SmlI 937 CTCAAG C^T[CT][GA]AG
Tth111I 928 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 681 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 545 CGCG CG^CG
636 CGCG
AcyI 466 GGCGTC G[GA]^CG[CT]C
1110 GGCGCC
AflIII 893 ACATGT A^C[GA][CT]GT
1139 ACATGT
AvaI 1049 CCCGGG C^[CT]CG[GA]G
1115 CCCGGG
BanI 675 GGTGCC G^G[CT][GA]CC
1110 GGCGCC
BbsI 100 GAAGAC GAAGAC(2/6)
1120 GTCTTC
BpuAI 100 GAAGAC GAAGAC(2/6)
1120 GTCTTC
BsaHI 466 GGCGTC G[GA]^CG[CT]C
1110 GGCGCC
BsgI 790 CTGCAC GTGCAG(16/14)
1305 CTGCAC
BsoBI 1049 CCCGGG C^[CT]CG[GA]G
1115 CCCGGG
BspLU11I 893 ACATGT A^CATGT
1139 ACATGT
BsrFI 865 ACCGGC [GA]^CCGG[CT]
1013 ACCGGC
BstUI 545 CGCG CG^CG
636 CGCG
BstYI 32 AGATCC [GA]^GATC[CT]
261 GGATCC
Cfr10I 865 ACCGGC [GA]^CCGG[CT]
1013 ACCGGC
HaeII 591 GGCGCT [GA]GCGC^[CT]
1110 GGCGCC
Hin1I 466 GGCGTC G[GA]^CG[CT]C
1110 GGCGCC
HincII 760 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HindII 760 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
MaeII 395 ACGT A^CGT
1145 ACGT
MboII 100 GAAGA GAAGA(8/7)
1121 TCTTC
MflI 32 AGATCC [GA]^GATC[CT]
261 GGATCC
MslI 615 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1140 CATGTACGTG
MvnI 545 CGCG CG^CG
636 CGCG
NspI 893 ACATGT [GA]CATG^[CT]
1139 ACATGT
PvuII 308 CAGCTG CAG^CTG
356 CAGCTG
SbfI 54 CCTGCAGG CCTGCA^GG
648 CCTGCAGG
SmaI 1049 CCCGGG CCC^GGG
1115 CCCGGG
Sse8387I 54 CCTGCAGG CCTGCA^GG
648 CCTGCAGG
XhoII 32 AGATCC [GA]^GATC[CT]
261 GGATCC
XmaCI 1049 CCCGGG C^CCGGG
1115 CCCGGG
XmaI 1049 CCCGGG C^CCGGG
1115 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI