Restriction map for
pF1KE0585
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# NO CUT ENZYMES ( 156 enzymes) #
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Acc65I AccIII AclI AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BcnI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaJI BseAI BseRI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssKI
BssSI Bst1107I BstEII BstNI BstPI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I FauI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvaI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 850 GACGTC GACGT^C
AccI 671 GTCTAC GT^[AC][GT]AC
AccII 124 CGCG CG^CG
AciI 125 GCGG CCGC(-3/-1)
AcsI 723 GAATTT [GA]^AATT[CT]
AcyI 850 GACGTC G[GA]^CG[CT]C
AflII 256 CTTAAG C^TTAAG
AflIII 605 ACATGT A^C[GA][CT]GT
AlwI 832 GATCC GGATC(4/5)
ApoI 723 GAATTT [GA]^AATT[CT]
BbrPI 224 CACGTG CAC^GTG
BciVI 634 GGATAC GTATCC(6/5)
BclI 365 TGATCA T^GATCA
BfaI 337 CTAG C^TAG
BfrI 256 CTTAAG C^TTAAG
BpmI 611 CTCCAG CTGGAG(16/14)
BsaAI 224 CACGTG [CT]AC^GT[GA]
BsaHI 850 GACGTC G[GA]^CG[CT]C
BsaI 659 GGTCTC GGTCTC(1/5)
BsaWI 69 ACCGGA [AT]^CCGG[AT]
BsiEI 67 CGACCG CG[GA][CT]^CG
BspLU11I 605 ACATGT A^CATGT
BsrFI 548 GCCGGT [GA]^CCGG[CT]
BstAPI 889 GCATGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 101 TTCGAA TT^CGAA
BstUI 124 CGCG CG^CG
BstXI 512 CCACTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 548 GCCGGT [GA]^CCGG[CT]
Cfr13I 28 GGGCC G^G[ACGT]CC
DdeI 41 CTCAG C^T[ACGT]AG
DraI 183 TTTAAA TTT^AAA
EaeI 546 TGGCCG [CT]^GGCC[GA]
FbaI 365 TGATCA T^GATCA
Hin1I 850 GACGTC G[GA]^CG[CT]C
HinfI 467 GAGTC G^A[ACGT]TC
MaeI 337 CTAG C^TAG
MspA1I 572 CAGCTG C[AC]G^C[GT]G
MvnI 124 CGCG CG^CG
NspV 101 TTCGAA TT^CGAA
PleI 467 GAGTC GAGTC(4/5)
PmaCI 224 CACGTG CAC^GTG
PmlI 224 CACGTG CAC^GTG
PvuII 572 CAGCTG CAG^CTG
Sau96I 28 GGGCC G^G[ACGT]CC
SfuI 101 TTCGAA TT^CGAA
SmlI 256 CTTAAG C^T[CT][GA]AG
SspI 287 AATATT AAT^ATT
StuI 59 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 172 GTAC GT^AC
593 GTAC
AhdI 652 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
845 GACCAGACGTC
AlwNI 341 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
509 CAGCCACTG
AspEI 652 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
845 GACCAGACGTC
BglII 249 AGATCT A^GATCT
534 AGATCT
BsgI 575 CTGCAC GTGCAG(16/14)
193 GTGCAG
BsiYI 70 CCGGATATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
703 CCTTTCTATGG
BslI 70 CCGGATATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
703 CCTTTCTATGG
BsmI 20 GAATGC GAATGC(1/-1)
456 GCATTC
BsrI 514 ACTGG ACTGG(1/-1)
175 CCAGT
Eam1105I 652 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
845 GACCAGACGTC
EarI 770 CTCTTC CTCTTC(1/4)
464 GAAGAG
HapII 70 CCGG C^CGG
549 CCGG
HpaII 70 CCGG C^CGG
549 CCGG
HphI 712 GGTGA GGTGA(8/7)
854 TCACC
Ksp632I 770 CTCTTC CTCTTC(1/4)
464 GAAGAG
MspI 70 CCGG C^CGG
549 CCGG
RsaI 172 GTAC GT^AC
593 GTAC
SphI 885 GCATGC GCATG^C
889 GCATGC
TaqI 102 TCGA T^CGA
462 TCGA
TthHB8I 102 TCGA T^CGA
462 TCGA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI BbvI BsmAI BstF5I BstYI
Cac8I DpnII EcoT22I Fnu4HI FokI
HaeIII ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MwoI NdeII NlaIII NsiI NspI
Sau3AI SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII