Restriction map for
pF1KE0584
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
StyI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AfaI 93 GTAC GT^AC
AspHI 361 GTGCTC G[AT]GC[AT]^C
AvaI 402 CCCGGG C^[CT]CG[GA]G
AvaII 11 GGACC G^G[AT]CC
BanII 292 GAGCCC G[GA]GC[CT]^C
BpmI 88 CTCCAG CTGGAG(16/14)
BsgI 172 CTGCAC GTGCAG(16/14)
BsiHKAI 361 GTGCTC G[AT]GC[AT]^C
BsoBI 402 CCCGGG C^[CT]CG[GA]G
BspMI 161 ACCTGC ACCTGC(4/8)
BsrI 90 CCAGT ACTGG(1/-1)
DdeI 447 CTCAG C^T[ACGT]AG
DraII 416 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 14 CCGTGG C^C[GA][CT]GG
EarI 331 GAAGAG CTCTTC(1/4)
EcoNI 464 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 416 GGGGCCT [GA]G^G[ACGT]CC[CT]
HinfI 115 GAGTC G^A[ACGT]TC
HphI 23 TCACC GGTGA(8/7)
Ksp632I 331 GAAGAG CTCTTC(1/4)
MslI 489 CACCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 453 CATG CATG^
PleI 115 GAGTC GAGTC(4/5)
PshAI 12 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 276 CAGCTG CAG^CTG
RsaI 93 GTAC GT^AC
ScaI 92 AGTACT AGT^ACT
SexAI 188 ACCTGGT A^CC[AT]GGT
SmaI 402 CCCGGG CCC^GGG
StuI 461 AGGCCT AGG^CCT
TspRI 286 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 179 CCAACCGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 402 CCCGGG C^CCGGG
XmaI 402 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 262 CGCG CG^CG
358 CGCG
AluI 277 AGCT AG^CT
395 AGCT
BmyI 292 GAGCCC G[AGT]GC[ACT]^C
361 GTGCTC
BsmFI 10 GGGAC GGGAC(10/14)
249 GTCCC
Bsp1286I 292 GAGCCC G[AGT]GC[ACT]^C
361 GTGCTC
BsrFI 83 GCCGGC [GA]^CCGG[CT]
207 GCCGGC
BstUI 262 CGCG CG^CG
358 CGCG
Cfr10I 83 GCCGGC [GA]^CCGG[CT]
207 GCCGGC
HaeII 210 GGCGCT [GA]GCGC^[CT]
426 GGCGCT
MboII 331 GAAGA GAAGA(8/7)
305 TCTTC
MspA1I 169 CCGCTG C[AC]G^C[GT]G
276 CAGCTG
MvnI 262 CGCG CG^CG
358 CGCG
NaeI 83 GCCGGC GCC^GGC
207 GCCGGC
NgoMIV 83 GCCGGC G^CCGGC
207 GCCGGC
PstI 310 CTGCAG CTGCA^G
421 CTGCAG
SbfI 309 CCTGCAGG CCTGCA^GG
420 CCTGCAGG
SfcI 310 CTGCAG C^T[GA][CT]AG
421 CTGCAG
Sse8387I 309 CCTGCAGG CCTGCA^GG
420 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI BbvI BcnI BsaJI BseRI
BsiYI BslI BssKI BstNI Cac8I
CfoI Cfr13I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MnlI MspI MvaI
MwoI NciI NlaIV Sau96I ScrFI
SfaNI TseI