Restriction map for
pF1KE0582
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 307 GCGG CCGC(-3/-1)
AocI 657 CCTAAGG CC^T[ACGT]AGG
AspHI 651 GTGCTC G[AT]GC[AT]^C
BalI 356 TGGCCA TGG^CCA
BclI 92 TGATCA T^GATCA
BfaI 698 CTAG C^TAG
BmyI 651 GTGCTC G[AGT]GC[ACT]^C
BpmI 538 CTGGAG CTGGAG(16/14)
BsgI 549 CTGCAC GTGCAG(16/14)
BsiHKAI 651 GTGCTC G[AT]GC[AT]^C
BsmFI 739 GTCCC GGGAC(10/14)
Bsp1286I 651 GTGCTC G[AGT]GC[ACT]^C
BspHI 389 TCATGA T^CATGA
BssKI 147 CCTGG ^CC[ACGT]GG
BstEII 106 GGTAACC G^GT[ACGT]ACC
BstNI 147 CCTGG CC^[AT]GG
BstPI 106 GGTAACC G^GT[ACGT]ACC
Bsu36I 657 CCTAAGG CC^T[ACGT]AGG
DraII 570 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 356 TGGCCA [CT]^GGCC[GA]
Eco81I 657 CCTAAGG CC^T[ACGT]AGG
EcoO109I 570 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 106 GGTAACC G^GT[ACGT]ACC
EcoRII 147 CCTGG ^CC[AT]GG
FbaI 92 TGATCA T^GATCA
HphI 83 TCACC GGTGA(8/7)
MaeI 698 CTAG C^TAG
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MspA1I 311 CAGCTG C[AC]G^C[GT]G
MvaI 147 CCTGG CC^[AT]GG
MwoI 885 GCTCTGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 484 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 279 GACTC GAGTC(4/5)
PstI 52 CTGCAG CTGCA^G
PvuII 311 CAGCTG CAG^CTG
RcaI 389 TCATGA T^CATGA
SapI 285 GCTCTTC GCTCTTC(1/4)
ScrFI 147 CCTGG CC^[ACGT]GG
SfcI 52 CTGCAG C^T[GA][CT]AG
TaqI 506 TCGA T^CGA
TthHB8I 506 TCGA T^CGA
Van91I 484 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 396 CCAATCTATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 162 GTAC GT^AC
529 GTAC
BsaI 316 GAGACC GGTCTC(1/5)
662 GGTCTC
BsaJI 146 CCCTGG C^C[ACGT][ACGT]GG
147 CCTGGG
BsmAI 316 GAGAC GTCTC(1/5)
663 GTCTC
BspMI 110 ACCTGC ACCTGC(4/8)
103 GCAGGT
BstF5I 21 CATCC GGATG(2/0)
627 GGATG
Cac8I 543 GCTGGC GC[ACGT]^[ACGT]GC
547 GCCTGC
EcoRI 16 GAATTC G^AATTC
431 GAATTC
FokI 21 CATCC GGATG(9/13)
627 GGATG
NlaIV 151 GGTTCC GG[ACGT]^[ACGT]CC
493 GGCCCC
RsaI 162 GTAC GT^AC
529 GTAC
SfaNI 628 GATGC GCATC(5/9)
681 GCATC
TfiI 410 GATTC G^A[AT]TC
455 GAATC
Tsp45I 597 GTCAC ^GT[GC]AC
796 GTGAC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsiYI
BslI BsrI Cfr13I DdeI DpnII
EarI Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI NdeII NlaIII Sau3AI Sau96I
Tru9I TseI Tsp509I TspRI