Restriction map for
pF1KE0580
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DdeI DraII DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 158 GACGCC G[GA]^CG[CT]C
AlwI 461 GGATC GGATC(4/5)
AspHI 189 GAGCAC G[AT]GC[AT]^C
BalI 213 TGGCCA TGG^CCA
BbsI 114 GAAGAC GAAGAC(2/6)
BbvI 79 GCAGC GCAGC(8/12)
BglI 70 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 189 GAGCAC G[AGT]GC[ACT]^C
BpuAI 114 GAAGAC GAAGAC(2/6)
BsaHI 158 GACGCC G[GA]^CG[CT]C
BsiEI 254 CGGCCG CG[GA][CT]^CG
BsiHKAI 189 GAGCAC G[AT]GC[AT]^C
Bsp1286I 189 GAGCAC G[AGT]GC[ACT]^C
BsrI 417 ACTGG ACTGG(1/-1)
BssKI 197 CCAGG ^CC[ACGT]GG
BstEII 369 GGTCACC G^GT[ACGT]ACC
BstNI 197 CCAGG CC^[AT]GG
BstPI 369 GGTCACC G^GT[ACGT]ACC
CfoI 4 GCGC GCG^C
Cfr13I 143 GGCCC G^G[ACGT]CC
DraI 63 TTTAAA TTT^AAA
DsaI 245 CCACGG C^C[GA][CT]GG
EagI 254 CGGCCG C^GGCCG
EclXI 254 CGGCCG C^GGCCG
Eco52I 254 CGGCCG C^GGCCG
EcoO65I 369 GGTCACC G^GT[ACGT]ACC
EcoRII 197 CCAGG ^CC[AT]GG
EcoT14I 181 CCTTGG C^C[AT][AT]GG
FauI 119 CCCGC CCCGC(4/6)
HaeII 3 GGCGCT [GA]GCGC^[CT]
HapII 162 CCGG C^CGG
HgaI 158 GACGC GACGC(5/10)
HhaI 4 GCGC GCG^C
Hin1I 158 GACGCC G[GA]^CG[CT]C
HinP1I 4 GCGC G^CGC
HindIII 208 AAGCTT A^AGCTT
HpaII 162 CCGG C^CGG
MaeII 124 ACGT A^CGT
MluNI 213 TGGCCA TGG^CCA
MscI 213 TGGCCA TGG^CCA
MspI 162 CCGG C^CGG
MvaI 197 CCAGG CC^[AT]GG
NlaIII 407 CATG CATG^
NlaIV 420 GGCTCC GG[ACGT]^[ACGT]CC
NspI 406 GCATGT [GA]CATG^[CT]
PleI 250 GAGTC GAGTC(4/5)
SapI 186 GAAGAGC GCTCTTC(1/4)
Sau96I 143 GGCCC G^G[ACGT]CC
ScrFI 197 CCAGG CC^[ACGT]GG
SfaNI 433 GATGC GCATC(5/9)
StyI 181 CCTTGG C^C[AT][AT]GG
TaqI 414 TCGA T^CGA
TfiI 381 GATTC G^A[AT]TC
TseI 79 GCAGC G^C[AT]GC
Tsp509I 456 AATT ^AATT
TthHB8I 414 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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BstF5I 300 GGATG GGATG(2/0)
441 GGATG
DpnII 324 GATC ^GATC
462 GATC
DrdI 117 GACCCGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
243 GACCACGGAGTC
EarI 186 GAAGAG CTCTTC(1/4)
261 GAAGAG
FokI 300 GGATG GGATG(9/13)
441 GGATG
HinfI 250 GAGTC G^A[ACGT]TC
381 GATTC
Ksp632I 186 GAAGAG CTCTTC(1/4)
261 GAAGAG
MaeIII 126 GTCAC ^GT[ACGT]AC
370 GTCAC
MboI 324 GATC ^GATC
462 GATC
MseI 64 TTAA T^TAA
305 TTAA
MspA1I 139 CAGCGG C[AC]G^C[GT]G
238 CCGCTG
NdeII 324 GATC ^GATC
462 GATC
Sau3AI 324 GATC ^GATC
462 GATC
Tru9I 64 TTAA T^TAA
305 TTAA
Tsp45I 126 GTCAC ^GT[GC]AC
370 GTCAC
TspRI 152 CACTG CA[GC]TG[ACGT][ACGT]^
294 CACTG
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AciI AluI BsaJI BsiYI BslI
EaeI Fnu4HI HaeIII HphI ItaI
MboII MnlI MwoI