Restriction map for
pF1KE0578
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BalI BanII BbvCI BcgI
BfrI BlpI Bpu1102I BsaWI BseAI
BsgI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeIII MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcsI 544 AAATTC [GA]^AATT[CT]
AflIII 388 ACACGT A^C[GA][CT]GT
Alw44I 241 GTGCAC G^TGCAC
ApaLI 241 GTGCAC G^TGCAC
ApoI 544 AAATTC [GA]^AATT[CT]
AspHI 241 GTGCAC G[AT]GC[AT]^C
AvaI 512 CCCGGG C^[CT]CG[GA]G
AvaII 248 GGACC G^G[AT]CC
AvrII 342 CCTAGG C^CTAGG
BamHI 173 GGATCC G^GATCC
BanI 182 GGCGCC G^G[CT][GA]CC
BbeI 182 GGCGCC GGCGC^C
BbrPI 389 CACGTG CAC^GTG
BbsI 19 GTCTTC GAAGAC(2/6)
BciVI 520 GGATAC GTATCC(6/5)
BclI 308 TGATCA T^GATCA
BfaI 343 CTAG C^TAG
BglI 200 GCCGCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 221 AGATCT A^GATCT
BlnI 342 CCTAGG C^CTAGG
BpuAI 19 GTCTTC GAAGAC(2/6)
BsaBI 309 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 602 GAGACC GGTCTC(1/5)
BsiHKAI 241 GTGCAC G[AT]GC[AT]^C
BsmBI 59 CGTCTC CGTCTC(1/5)
BsmFI 247 GGGAC GGGAC(10/14)
BsoBI 512 CCCGGG C^[CT]CG[GA]G
Bsp1407I 452 TGTACA T^GTACA
BsrFI 205 ACCGGC [GA]^CCGG[CT]
BsrGI 452 TGTACA T^GTACA
BstF5I 315 CATCC GGATG(2/0)
Cac8I 188 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 205 ACCGGC [GA]^CCGG[CT]
DraII 247 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 247 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 251 CCCGC CCCGC(4/6)
FbaI 308 TGATCA T^GATCA
FokI 315 CATCC GGATG(9/13)
HgaI 17 GCGTC GACGC(5/10)
KasI 182 GGCGCC G^GCGCC
MaeI 343 CTAG C^TAG
MamI 309 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 389 CACGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 182 GGCGCC GG^CGCC
NlaIII 594 CATG CATG^
PmaCI 389 CACGTG CAC^GTG
PmlI 389 CACGTG CAC^GTG
PpuMI 247 GGGACCC [GA]G^G[AT]CC[CT]
PstI 133 CTGCAG CTGCA^G
SfaNI 314 GCATC GCATC(5/9)
SfoI 182 GGCGCC GGC^GCC
SmaI 512 CCCGGG CCC^GGG
SspBI 452 TGTACA T^GTACA
StuI 565 AGGCCT AGG^CCT
TfiI 275 GAATC G^A[AT]TC
Tsp509I 545 AATT ^AATT
XmaCI 512 CCCGGG C^CCGGG
XmaI 512 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 16 GGCGTC G[GA]^CG[CT]C
182 GGCGCC
BbvI 135 GCAGC GCAGC(8/12)
132 GCTGC
BmyI 73 GTGCCC G[AGT]GC[ACT]^C
241 GTGCAC
BpmI 217 CTGGAG CTGGAG(16/14)
280 CTGGAG
BsaAI 389 CACGTG [CT]AC^GT[GA]
484 TACGTG
BsaHI 16 GGCGTC G[GA]^CG[CT]C
182 GGCGCC
BseRI 54 CTCCTC GAGGAG(10/8)
359 GAGGAG
Bsp1286I 73 GTGCCC G[AGT]GC[ACT]^C
241 GTGCAC
BsrI 35 ACTGG ACTGG(1/-1)
425 ACTGG
CfoI 108 GCGC GCG^C
183 GCGC
EcoT14I 29 CCTTGG C^C[AT][AT]GG
342 CCTAGG
HaeII 107 AGCGCC [GA]GCGC^[CT]
182 GGCGCC
HhaI 108 GCGC GCG^C
183 GCGC
Hin1I 16 GGCGTC G[GA]^CG[CT]C
182 GGCGCC
HinP1I 108 GCGC G^CGC
183 GCGC
HphI 145 TCACC GGTGA(8/7)
508 TCACC
MwoI 183 GCGCCGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
200 GCCGCACCGGC
StyI 29 CCTTGG C^C[AT][AT]GG
342 CCTAGG
TaqI 122 TCGA T^CGA
376 TCGA
TseI 132 GCTGC G^C[AT]GC
135 GCAGC
TthHB8I 122 TCGA T^CGA
376 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI BcnI
BsaJI BsiYI BslI BsmAI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MaeII MboI MboII
MflI MnlI MspI MvaI NciI
NdeII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI XhoII