Restriction map for
pF1KE0576
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AluI 91 AGCT AG^CT
AlwI 548 GGATC GGATC(4/5)
AocI 441 CCTCAGG CC^T[ACGT]AGG
ApaI 461 GGGCCC GGGCC^C
BanII 461 GGGCCC G[GA]GC[CT]^C
BlpI 361 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 361 GCTGAGC GC^T[ACGT]AGC
BsaI 628 GAGACC GGTCTC(1/5)
BsaWI 218 TCCGGT [AT]^CCGG[AT]
BseRI 439 CTCCTC GAGGAG(10/8)
BsmFI 149 GGGAC GGGAC(10/14)
BstAPI 604 GCACTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 345 GGTGACC G^GT[ACGT]ACC
BstPI 345 GGTGACC G^GT[ACGT]ACC
BstXI 350 CCAAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 548 GGATCT [GA]^GATC[CT]
Bsu36I 441 CCTCAGG CC^T[ACGT]AGG
CelII 361 GCTGAGC GC^T[ACGT]AGC
CfoI 288 GCGC GCG^C
DraII 460 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 151 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 560 CCATGG C^C[GA][CT]GG
EarI 685 CTCTTC CTCTTC(1/4)
Eco81I 441 CCTCAGG CC^T[ACGT]AGG
EcoO109I 460 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 345 GGTGACC G^GT[ACGT]ACC
EcoT22I 776 ATGCAT ATGCA^T
FauI 398 CCCGC CCCGC(4/6)
HaeII 287 GGCGCT [GA]GCGC^[CT]
HhaI 288 GCGC GCG^C
HinP1I 288 GCGC G^CGC
Ksp632I 685 CTCTTC CTCTTC(1/4)
MaeII 186 ACGT A^CGT
MflI 548 GGATCT [GA]^GATC[CT]
MslI 367 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 560 CCATGG C^CATGG
NsiI 776 ATGCAT ATGCA^T
PstI 587 CTGCAG CTGCA^G
SbfI 586 CCTGCAGG CCTGCA^GG
SexAI 301 ACCTGGT A^CC[AT]GGT
SfaNI 778 GCATC GCATC(5/9)
SfcI 587 CTGCAG C^T[GA][CT]AG
Sse8387I 586 CCTGCAGG CCTGCA^GG
StuI 634 AGGCCT AGG^CCT
TspRI 605 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 742 CCATTCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 548 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 352 AAATTC [GA]^AATT[CT]
760 AAATTC
AfaI 175 GTAC GT^AC
756 GTAC
ApoI 352 AAATTC [GA]^AATT[CT]
760 AAATTC
AspHI 364 GAGCAC G[AT]GC[AT]^C
436 GTGCTC
BbvI 582 GCAGC GCAGC(8/12)
447 GCTGC
BcnI 171 CCCGG CC^[GC]GG
414 CCGGG
BsgI 784 CTGCAC GTGCAG(16/14)
498 GTGCAG
BsiHKAI 364 GAGCAC G[AT]GC[AT]^C
436 GTGCTC
BsmAI 41 GAGAC GTCTC(1/5)
628 GAGAC
BsrBI 132 CCGCTC CCGCTC(-3/-3)
28 GAGCGG
BsrDI 554 GCAATG GCAATG(2/0)
728 GCAATG
EcoT14I 560 CCATGG C^C[AT][AT]GG
751 CCTTGG
MaeIII 346 GTGAC ^GT[ACGT]AC
376 GTCAC
MboII 471 GAAGA GAAGA(8/7)
686 TCTTC
NciI 171 CCCGG CC^[GC]GG
414 CCGGG
RsaI 175 GTAC GT^AC
756 GTAC
StyI 560 CCATGG C^C[AT][AT]GG
751 CCTTGG
TseI 447 GCTGC G^C[AT]GC
582 GCAGC
Tsp45I 346 GTGAC ^GT[GC]AC
376 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI TfiI
Tsp509I TthHB8I