Restriction map for
pF1KE0574
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AciI AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MspA1I
MunI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 446 TCCGGA T^CCGGA
AcyI 322 GGCGTC G[GA]^CG[CT]C
AlwI 56 GGATC GGATC(4/5)
AlwNI 141 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 444 GGTCC G^G[AT]CC
BbsI 842 GTCTTC GAAGAC(2/6)
BbvI 256 GCTGC GCAGC(8/12)
BpuAI 842 GTCTTC GAAGAC(2/6)
BsaHI 322 GGCGTC G[GA]^CG[CT]C
BseAI 446 TCCGGA T^CCGGA
BsmFI 716 GTCCC GGGAC(10/14)
BspEI 446 TCCGGA T^CCGGA
BspMI 430 ACCTGC ACCTGC(4/8)
BsrDI 830 GCAATG GCAATG(2/0)
BstAPI 250 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 782 TTCGAA TT^CGAA
BstXI 615 CCATTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 444 GGTCC G^G[ACGT]CC
DdeI 632 CTGAG C^T[ACGT]AG
Fnu4HI 256 GCTGC GC^[ACGT]GC
Hin1I 322 GGCGTC G[GA]^CG[CT]C
HincII 289 GTCAAC GT[CT]^[GA]AC
HindII 289 GTCAAC GT[CT]^[GA]AC
ItaI 256 GCTGC GC^[ACGT]GC
MaeIII 29 GTGAC ^GT[ACGT]AC
MroI 446 TCCGGA T^CCGGA
NcoI 759 CCATGG C^CATGG
NspV 782 TTCGAA TT^CGAA
Sau96I 444 GGTCC G^G[ACGT]CC
SfuI 782 TTCGAA TT^CGAA
TaqI 783 TCGA T^CGA
TseI 256 GCTGC G^C[AT]GC
Tsp45I 29 GTGAC ^GT[GC]AC
TthHB8I 783 TCGA T^CGA
XbaI 399 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 60 CGCG CG^CG
499 CGCG
AluI 235 AGCT AG^CT
582 AGCT
BfaI 163 CTAG C^TAG
400 CTAG
BsaWI 47 TCCGGT [AT]^CCGG[AT]
446 TCCGGA
BsiYI 435 CCTTCGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
718 CCCTCTTGTGG
BslI 435 CCTTCGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
718 CCCTCTTGTGG
BssKI 228 CCAGG ^CC[ACGT]GG
348 CCAGG
BstF5I 675 CATCC GGATG(2/0)
703 CATCC
BstNI 228 CCAGG CC^[AT]GG
348 CCAGG
BstUI 60 CGCG CG^CG
499 CGCG
DpnII 57 GATC ^GATC
397 GATC
DsaI 37 CCACGG C^C[GA][CT]GG
759 CCATGG
EarI 284 GAAGAG CTCTTC(1/4)
511 GAAGAG
EcoRII 228 CCAGG ^CC[AT]GG
348 CCAGG
EcoT14I 621 CCTTGG C^C[AT][AT]GG
759 CCATGG
FokI 675 CATCC GGATG(9/13)
703 CATCC
HapII 48 CCGG C^CGG
447 CCGG
HgaI 61 GCGTC GACGC(5/10)
323 GCGTC
HpaII 48 CCGG C^CGG
447 CCGG
Ksp632I 284 GAAGAG CTCTTC(1/4)
511 GAAGAG
MaeI 163 CTAG C^TAG
400 CTAG
MboI 57 GATC ^GATC
397 GATC
MslI 703 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
707 CACATGAGTG
MspI 48 CCGG C^CGG
447 CCGG
MvaI 228 CCAGG CC^[AT]GG
348 CCAGG
MvnI 60 CGCG CG^CG
499 CGCG
NdeII 57 GATC ^GATC
397 GATC
Sau3AI 57 GATC ^GATC
397 GATC
ScrFI 228 CCAGG CC^[ACGT]GG
348 CCAGG
SfaNI 563 GATGC GCATC(5/9)
702 GCATC
StyI 621 CCTTGG C^C[AT][AT]GG
759 CCATGG
TspRI 72 CAGTG CA[GC]TG[ACGT][ACGT]^
261 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AcsI AfaI ApoI BalI BsaJI
BsrI Cac8I EaeI HaeIII HinfI
HphI MboII MluNI MnlI MscI
MseI MwoI NlaIII PleI RsaI
TfiI Tru9I Tsp509I