Restriction map for
pF1KE0571
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII Bst1107I BstBI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI Sau3AI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI StuI
StyI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AspHI 26 GTGCTC G[AT]GC[AT]^C
AvaII 307 GGACC G^G[AT]CC
BanI 101 GGCACC G^G[CT][GA]CC
BanII 80 GAGCCC G[GA]GC[CT]^C
BbsI 472 GTCTTC GAAGAC(2/6)
BglI 508 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 472 GTCTTC GAAGAC(2/6)
BseRI 77 GAGGAG GAGGAG(10/8)
BsiHKAI 26 GTGCTC G[AT]GC[AT]^C
BsmAI 55 GAGAC GTCTC(1/5)
BsrDI 396 CATTGC GCAATG(2/0)
BssSI 388 CTCGTG CACGAG(-5/-1)
BstAPI 102 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 336 GGTCACC G^GT[ACGT]ACC
BstF5I 311 CATCC GGATG(2/0)
BstPI 336 GGTCACC G^GT[ACGT]ACC
BstXI 461 CCACCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 59 CTCAG C^T[ACGT]AG
DraII 544 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 103 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 228 GAAGAG CTCTTC(1/4)
EcoNI 411 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 544 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 336 GGTCACC G^GT[ACGT]ACC
FauI 14 CCCGC CCCGC(4/6)
FokI 311 CATCC GGATG(9/13)
HphI 338 TCACC GGTGA(8/7)
Ksp632I 228 GAAGAG CTCTTC(1/4)
MaeII 254 ACGT A^CGT
MaeIII 337 GTCAC ^GT[ACGT]AC
MspA1I 19 CAGCTG C[AC]G^C[GT]G
NspI 245 GCATGC [GA]CATG^[CT]
PstI 364 CTGCAG CTGCA^G
PvuII 19 CAGCTG CAG^CTG
SapI 228 GAAGAGC GCTCTTC(1/4)
SbfI 363 CCTGCAGG CCTGCA^GG
SfcI 364 CTGCAG C^T[GA][CT]AG
SphI 245 GCATGC GCATG^C
Sse8387I 363 CCTGCAGG CCTGCA^GG
TaqI 161 TCGA T^CGA
Tsp45I 337 GTCAC ^GT[GC]AC
TthHB8I 161 TCGA T^CGA
XcmI 224 CCAAGAAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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BcnI 98 CCCGG CC^[GC]GG
105 CCCGG
BpmI 74 CTGGAG CTGGAG(16/14)
298 CTGGAG
BspMI 4 GCAGGT ACCTGC(4/8)
366 GCAGGT
HaeIII 258 GGCC GG^CC
546 GGCC
HapII 99 CCGG C^CGG
106 CCGG
HpaII 99 CCGG C^CGG
106 CCGG
MspI 99 CCGG C^CGG
106 CCGG
NciI 98 CCCGG CC^[GC]GG
105 CCCGG
PleI 57 GACTC GAGTC(4/5)
437 GACTC
SmlI 124 CTTGAG C^T[CT][GA]AG
549 CTCAAG
TfiI 142 GAATC G^A[AT]TC
555 GAATC
TspRI 430 CAGTG CA[GC]TG[ACGT][ACGT]^
480 CACTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I Cfr13I EcoRII Fnu4HI
HinfI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI