Restriction map for
pF1KE0570
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AfaI 277 GTAC GT^AC
AlwI 409 GGATC GGATC(4/5)
AocI 647 CCTCAGG CC^T[ACGT]AGG
AspHI 629 GAGCAC G[AT]GC[AT]^C
AvaI 68 CCCGGG C^[CT]CG[GA]G
BalI 533 TGGCCA TGG^CCA
BfaI 74 CTAG C^TAG
BmyI 629 GAGCAC G[AGT]GC[ACT]^C
BseRI 700 CTCCTC GAGGAG(10/8)
BsgI 623 GTGCAG GTGCAG(16/14)
BsiHKAI 629 GAGCAC G[AT]GC[AT]^C
BsmBI 80 GAGACG CGTCTC(1/5)
BsoBI 68 CCCGGG C^[CT]CG[GA]G
Bsp1286I 629 GAGCAC G[AGT]GC[ACT]^C
BspDI 427 ATCGAT AT^CGAT
BsrI 274 CCAGT ACTGG(1/-1)
BstYI 409 GGATCT [GA]^GATC[CT]
Bsu36I 647 CCTCAGG CC^T[ACGT]AGG
Cac8I 363 GCAGGC GC[ACGT]^[ACGT]GC
ClaI 427 ATCGAT AT^CGAT
DpnII 410 GATC ^GATC
DrdI 106 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 139 CCACGG C^C[GA][CT]GG
EaeI 533 TGGCCA [CT]^GGCC[GA]
EarI 462 GAAGAG CTCTTC(1/4)
Eco81I 647 CCTCAGG CC^T[ACGT]AGG
EcoT14I 344 CCAAGG C^C[AT][AT]GG
FauI 39 CCCGC CCCGC(4/6)
HapII 69 CCGG C^CGG
HinfI 454 GACTC G^A[ACGT]TC
HpaII 69 CCGG C^CGG
Ksp632I 462 GAAGAG CTCTTC(1/4)
MaeI 74 CTAG C^TAG
MboI 410 GATC ^GATC
MflI 409 GGATCT [GA]^GATC[CT]
MluNI 533 TGGCCA TGG^CCA
MscI 533 TGGCCA TGG^CCA
MspA1I 325 CAGCTG C[AC]G^C[GT]G
MspI 69 CCGG C^CGG
NdeII 410 GATC ^GATC
PleI 454 GACTC GAGTC(4/5)
PstI 361 CTGCAG CTGCA^G
PvuII 325 CAGCTG CAG^CTG
RsaI 277 GTAC GT^AC
Sau3AI 410 GATC ^GATC
SbfI 360 CCTGCAGG CCTGCA^GG
ScaI 276 AGTACT AGT^ACT
SfaNI 734 GCATC GCATC(5/9)
SfcI 361 CTGCAG C^T[GA][CT]AG
SmaI 68 CCCGGG CCC^GGG
SmlI 580 CTTGAG C^T[CT][GA]AG
Sse8387I 360 CCTGCAGG CCTGCA^GG
StyI 344 CCAAGG C^C[AT][AT]GG
TaqI 428 TCGA T^CGA
Tsp509I 334 AATT ^AATT
TspRI 405 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 428 TCGA T^CGA
XhoII 409 GGATCT [GA]^GATC[CT]
XmaCI 68 CCCGGG C^CCGGG
XmaI 68 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AvaII 65 GGTCC G^G[AT]CC
136 GGACC
BcnI 68 CCCGG CC^[GC]GG
69 CCGGG
BsmAI 80 GAGAC GTCTC(1/5)
563 GAGAC
BsmFI 66 GTCCC GGGAC(10/14)
224 GTCCC
HphI 110 TCACC GGTGA(8/7)
356 TCACC
MaeIII 211 GTCAC ^GT[ACGT]AC
247 GTCAC
NciI 68 CCCGG CC^[GC]GG
69 CCGGG
Tsp45I 211 GTCAC ^GT[GC]AC
247 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I DdeI EcoRII Fnu4HI FokI
HaeIII ItaI MboII MnlI MslI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TseI