##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       Acc65I      AgeI        AscI        Asp718I     
AviII       AvrII       BbeI        BbrPI       BlnI        
BsiEI       BsiWI       BspDI       BsrFI       BssHII      
Bst1107I    BstZ17I     Cfr10I      ClaI        EagI        
EclXI       Eco52I      FseI        FspI        KasI        
KpnI        MfeI        MluI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PvuI        SalI        SbfI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SwaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         6574  GTAGAC               GT^[AC][GT]AC
AccIII       4682  TCCGGA               T^CCGGA
AclI         6284  AACGTT               AA^CGTT
AcyI          603  GACGCC               G[GA]^CG[CT]C
AflII        2971  CTTAAG               C^TTAAG
Aor51HI      7609  AGCGCT               AGC^GCT
ApaI         3620  GGGCCC               GGGCC^C
AseI         5082  ATTAAT               AT^TAAT
AsnI         5082  ATTAAT               AT^TAAT
AspI         1345  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BfrI         2971  CTTAAG               C^TTAAG
BsaAI        6710  TACGTG               [CT]AC^GT[GA]
BsaHI         603  GACGCC               G[GA]^CG[CT]C
BseAI        4682  TCCGGA               T^CCGGA
BspEI        4682  TCCGGA               T^CCGGA
BssSI        7832  CTCGTG               CACGAG(-5/-1)
BstBI        2493  TTCGAA               TT^CGAA
BstEII       2793  GGTGACC              G^GT[ACGT]ACC
BstPI        2793  GGTGACC              G^GT[ACGT]ACC
CpoI         5635  CGGTCCG              CG^G[AT]CCG
DrdI         1558  GACGTGCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     7609  AGCGCT               AGC^GCT
EcoO65I      2793  GGTGACC              G^GT[ACGT]ACC
EcoRV        4399  GATATC               GAT^ATC
EcoT22I       502  ATGCAT               ATGCA^T
Hin1I         603  GACGCC               G[GA]^CG[CT]C
HindIII      6703  AAGCTT               A^AGCTT
HpaI         8337  GTTAAC               GTT^AAC
KspI          567  CCGCGG               CCGC^GG
MroI         4682  TCCGGA               T^CCGGA
NdeI         6299  CATATG               CA^TATG
NsiI          502  ATGCAT               ATGCA^T
NspV         2493  TTCGAA               TT^CGAA
PaeR7I       7795  CTCGAG               C^TCGAG
PflFI        1345  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        8059  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        5082  ATTAAT               AT^TAAT
Psp1406I     6284  AACGTT               AA^CGTT
RsrII        5635  CGGTCCG              CG^G[AT]CCG
SacII         567  CCGCGG               CCGC^GG
SexAI        7538  ACCTGGT              A^CC[AT]GGT
SfiI         7860  GGCCCTCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         2493  TTCGAA               TT^CGAA
SpeI         3232  ACTAGT               A^CTAGT
Tth111I      1345  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         7795  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AflIII       3830  ACATGT               A^C[GA][CT]GT
             5765  ACATGT              
Alw44I        959  GTGCAC               G^TGCAC
             1561  GTGCAC              
ApaLI         959  GTGCAC               G^TGCAC
             1561  GTGCAC              
BcgI          660  CGAGGAGAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2028  CGATTTGGTTGC        
BspLU11I     3830  ACATGT               A^CATGT
             5765  ACATGT              
NcoI         3509  CCATGG               C^CATGG
             4539  CCATGG              
XbaI         4818  TCTAGA               T^CTAGA
             4869  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  153 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AhdI        
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AspEI       AspHI       AvaI        AvaII       
BalI        BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BciVI       BclI        BcnI        
BfaI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspHI       
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        Eam1105I    
EarI        Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        SacI        SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI