Restriction map for
pF1KE0479
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbsI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HapII
HgaI Hin1I HpaI HpaII KasI
KpnI KspI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tth111I TthHB8I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AciI 471 GCGG CCGC(-3/-1)
AcsI 292 AAATTT [GA]^AATT[CT]
AflIII 647 ACATGT A^C[GA][CT]GT
Alw44I 36 GTGCAC G^TGCAC
AlwNI 422 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 544 CCTGAGG CC^T[ACGT]AGG
Aor51HI 305 AGCGCT AGC^GCT
ApaLI 36 GTGCAC G^TGCAC
ApoI 292 AAATTT [GA]^AATT[CT]
BalI 141 TGGCCA TGG^CCA
BamHI 770 GGATCC G^GATCC
BanI 812 GGCACC G^G[CT][GA]CC
BanII 274 GAGCTC G[GA]GC[CT]^C
BbvCI 94 CCTCAGC CCTCAGC(-5/-2)
BbvI 896 GCAGC GCAGC(8/12)
BfaI 628 CTAG C^TAG
BsaBI 418 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 538 CTGCAC GTGCAG(16/14)
BsmFI 30 GTCCC GGGAC(10/14)
BsmI 606 GAATGC GAATGC(1/-1)
BspHI 87 TCATGA T^CATGA
BspLU11I 647 ACATGT A^CATGT
BspMI 5 ACCTGC ACCTGC(4/8)
BsrBI 469 GAGCGG CCGCTC(-3/-3)
BsrDI 507 CATTGC GCAATG(2/0)
BstXI 809 CCAGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 544 CCTGAGG CC^T[ACGT]AGG
Cac8I 848 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 306 GCGC GCG^C
DraI 478 TTTAAA TTT^AAA
DrdI 253 GACGGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 141 TGGCCA [CT]^GGCC[GA]
Eco47III 305 AGCGCT AGC^GCT
Eco81I 544 CCTGAGG CC^T[ACGT]AGG
EcoNI 351 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 896 GCAGC GC^[ACGT]GC
HaeII 305 AGCGCT [GA]GCGC^[CT]
HhaI 306 GCGC GCG^C
HinP1I 306 GCGC G^CGC
HincII 871 GTTGAC GT[CT]^[GA]AC
HindII 871 GTTGAC GT[CT]^[GA]AC
HindIII 850 AAGCTT A^AGCTT
HinfI 826 GAATC G^A[ACGT]TC
HphI 643 GGTGA GGTGA(8/7)
ItaI 896 GCAGC GC^[ACGT]GC
MaeI 628 CTAG C^TAG
MaeII 407 ACGT A^CGT
MamI 418 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 141 TGGCCA TGG^CCA
MscI 141 TGGCCA TGG^CCA
MslI 115 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 187 CAGCTG C[AC]G^C[GT]G
MwoI 163 GCTGAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 647 ACATGT [GA]CATG^[CT]
PflMI 761 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 187 CAGCTG CAG^CTG
RcaI 87 TCATGA T^CATGA
SacI 274 GAGCTC GAGCT^C
SexAI 815 ACCTGGT A^CC[AT]GGT
SmlI 51 CTTGAG C^T[CT][GA]AG
StuI 920 AGGCCT AGG^CCT
TfiI 826 GAATC G^A[AT]TC
TseI 896 GCAGC G^C[AT]GC
Tsp509I 293 AATT ^AATT
Van91I 761 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 627 TCTAGA T^CTAGA
XcmI 834 CCAAAACAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 36 GTGCAC G[AT]GC[AT]^C
274 GAGCTC
AvaII 3 GGACC G^G[AT]CC
75 GGACC
BclI 183 TGATCA T^GATCA
789 TGATCA
BmyI 36 GTGCAC G[AGT]GC[ACT]^C
274 GAGCTC
BsaI 500 GAGACC GGTCTC(1/5)
757 GAGACC
BsiHKAI 36 GTGCAC G[AT]GC[AT]^C
274 GAGCTC
BsmAI 500 GAGAC GTCTC(1/5)
757 GAGAC
Bsp1286I 36 GTGCAC G[AGT]GC[ACT]^C
274 GAGCTC
Bsp1407I 81 TGTACA T^GTACA
650 TGTACA
BsrGI 81 TGTACA T^GTACA
650 TGTACA
BsrI 284 ACTGG ACTGG(1/-1)
767 ACTGG
BstYI 423 AGATCC [GA]^GATC[CT]
770 GGATCC
Cfr13I 3 GGACC G^G[ACGT]CC
75 GGACC
EcoT14I 515 CCAAGG C^C[AT][AT]GG
857 CCAAGG
FbaI 183 TGATCA T^GATCA
789 TGATCA
MflI 423 AGATCC [GA]^GATC[CT]
770 GGATCC
NlaIV 770 GGATCC GG[ACGT]^[ACGT]CC
812 GGCACC
Sau96I 3 GGACC G^G[ACGT]CC
75 GGACC
SfaNI 121 GATGC GCATC(5/9)
148 GATGC
SspBI 81 TGTACA T^GTACA
650 TGTACA
StyI 515 CCAAGG C^C[AT][AT]GG
857 CCAAGG
XhoII 423 AGATCC [GA]^GATC[CT]
770 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BseRI
BsiYI BslI BssKI BstF5I BstNI
DdeI DpnII EarI EcoRII FokI
HaeIII Ksp632I MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI Tru9I Tsp45I
TspRI