Restriction map for
pF1KE0458
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 459 GTAC GT^AC
Aor51HI 279 AGCGCT AGC^GCT
AspHI 553 GAGCAC G[AT]GC[AT]^C
AvaI 148 CTCGGG C^[CT]CG[GA]G
BanI 520 GGCACC G^G[CT][GA]CC
BanII 61 GGGCTC G[GA]GC[CT]^C
BbsI 415 GTCTTC GAAGAC(2/6)
BcnI 10 CCCGG CC^[GC]GG
BfaI 325 CTAG C^TAG
BlpI 613 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 613 GCTCAGC GC^T[ACGT]AGC
BpuAI 415 GTCTTC GAAGAC(2/6)
BsgI 507 GTGCAG GTGCAG(16/14)
BsiHKAI 553 GAGCAC G[AT]GC[AT]^C
BsoBI 148 CTCGGG C^[CT]CG[GA]G
BssHII 160 GCGCGC G^CGCGC
BstAPI 579 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 524 CCATCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 613 GCTCAGC GC^T[ACGT]AGC
DrdI 406 GACCATCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 87 GAAGAG CTCTTC(1/4)
Eco47III 279 AGCGCT AGC^GCT
EcoRV 451 GATATC GAT^ATC
EcoT14I 516 CCATGG C^C[AT][AT]GG
HaeII 279 AGCGCT [GA]GCGC^[CT]
HapII 11 CCGG C^CGG
HgaI 172 GACGC GACGC(5/10)
HincII 637 GTCAAC GT[CT]^[GA]AC
HindII 637 GTCAAC GT[CT]^[GA]AC
HpaII 11 CCGG C^CGG
HphI 234 GGTGA GGTGA(8/7)
Ksp632I 87 GAAGAG CTCTTC(1/4)
KspI 49 CCGCGG CCGC^GG
MaeI 325 CTAG C^TAG
MaeII 344 ACGT A^CGT
MaeIII 298 GTTAC ^GT[ACGT]AC
MspI 11 CCGG C^CGG
NciI 10 CCCGG CC^[GC]GG
NcoI 516 CCATGG C^CATGG
NspI 111 GCATGT [GA]CATG^[CT]
PstI 283 CTGCAG CTGCA^G
PvuII 362 CAGCTG CAG^CTG
RsaI 459 GTAC GT^AC
SacII 49 CCGCGG CCGC^GG
SapI 87 GAAGAGC GCTCTTC(1/4)
SexAI 434 ACCTGGT A^CC[AT]GGT
SfaNI 260 GCATC GCATC(5/9)
StyI 516 CCATGG C^C[AT][AT]GG
Tsp509I 193 AATT ^AATT
TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 13 enzymes) #
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BcgI 240 CGAACTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
191 GCAATTATTTCG
BmyI 61 GGGCTC G[AGT]GC[ACT]^C
553 GAGCAC
BpmI 365 CTGGAG CTGGAG(16/14)
604 CTGGAG
Bsp1286I 61 GGGCTC G[AGT]GC[ACT]^C
553 GAGCAC
BspMI 311 ACCTGC ACCTGC(4/8)
672 ACCTGC
DsaI 49 CCGCGG C^C[GA][CT]GG
516 CCATGG
MboII 87 GAAGA GAAGA(8/7)
416 TCTTC
MwoI 579 GCACCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
609 GCTGGCTCAGC
PpuMI 139 GGGTCCC [GA]G^G[AT]CC[CT]
490 GGGACCC
SfcI 283 CTGCAG C^T[GA][CT]AG
645 CTATAG
TaqI 200 TCGA T^CGA
239 TCGA
TfiI 25 GAATC G^A[AT]TC
37 GAATC
TthHB8I 200 TCGA T^CGA
239 TCGA
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI ItaI MboI MflI MnlI
MspA1I MvaI MvnI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
TseI XhoII