Restriction map for
pF1KE0456
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaII AviII AvrII BamHI
BbsI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 72 enzymes) #
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AccII 365 CGCG CG^CG
AcyI 647 GGCGCC G[GA]^CG[CT]C
AlwI 528 GATCC GGATC(4/5)
AlwNI 70 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 578 GGGCCC GGGCC^C
AspHI 397 GTGCTC G[AT]GC[AT]^C
AspI 103 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 342 CCCGGG C^[CT]CG[GA]G
BalI 683 TGGCCA TGG^CCA
BanI 647 GGCGCC G^G[CT][GA]CC
BbeI 647 GGCGCC GGCGC^C
BbrPI 351 CACGTG CAC^GTG
BbvCI 730 CCTCAGC CCTCAGC(-5/-2)
BcgI 503 GCACGCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 591 GCTCAGC GC^T[ACGT]AGC
BpmI 67 CTCCAG CTGGAG(16/14)
Bpu1102I 591 GCTCAGC GC^T[ACGT]AGC
BsaAI 351 CACGTG [CT]AC^GT[GA]
BsaBI 528 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 647 GGCGCC G[GA]^CG[CT]C
BsiEI 793 CGGCCG CG[GA][CT]^CG
BsiHKAI 397 GTGCTC G[AT]GC[AT]^C
BsmBI 174 CGTCTC CGTCTC(1/5)
BsmFI 320 GGGAC GGGAC(10/14)
BsoBI 342 CCCGGG C^[CT]CG[GA]G
BspMI 374 ACCTGC ACCTGC(4/8)
BstAPI 552 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 365 CGCG CG^CG
BstXI 413 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 527 AGATCC [GA]^GATC[CT]
CelII 591 GCTCAGC GC^T[ACGT]AGC
DpnII 528 GATC ^GATC
DraIII 565 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 793 CGGCCG C^GGCCG
EclXI 793 CGGCCG C^GGCCG
Eco52I 793 CGGCCG C^GGCCG
EcoNI 375 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 785 CCCGC CCCGC(4/6)
HgaI 103 GACGC GACGC(5/10)
Hin1I 647 GGCGCC G[GA]^CG[CT]C
HincII 700 GTCAAC GT[CT]^[GA]AC
HindII 700 GTCAAC GT[CT]^[GA]AC
HinfI 41 GACTC G^A[ACGT]TC
KasI 647 GGCGCC G^GCGCC
MaeIII 284 GTCAC ^GT[ACGT]AC
MamI 528 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 528 GATC ^GATC
MflI 527 AGATCC [GA]^GATC[CT]
MluNI 683 TGGCCA TGG^CCA
MscI 683 TGGCCA TGG^CCA
MslI 483 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 70 CAGCGG C[AC]G^C[GT]G
MvnI 365 CGCG CG^CG
NarI 647 GGCGCC GG^CGCC
NcoI 413 CCATGG C^CATGG
NdeII 528 GATC ^GATC
NspI 482 GCATGT [GA]CATG^[CT]
PflFI 103 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 41 GACTC GAGTC(4/5)
PmaCI 351 CACGTG CAC^GTG
PmlI 351 CACGTG CAC^GTG
Sau3AI 528 GATC ^GATC
SexAI 299 ACCAGGT A^CC[AT]GGT
SfaNI 585 GATGC GCATC(5/9)
SfoI 647 GGCGCC GGC^GCC
SmaI 342 CCCGGG CCC^GGG
Tsp45I 284 GTCAC ^GT[GC]AC
Tsp509I 236 AATT ^AATT
Tth111I 103 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 527 AGATCC [GA]^GATC[CT]
XmaCI 342 CCCGGG C^CCGGG
XmaI 342 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 540 GTAC GT^AC
633 GTAC
BanII 338 GAGCCC G[GA]GC[CT]^C
578 GGGCCC
BseRI 49 CTCCTC GAGGAG(10/8)
94 GAGGAG
BsgI 376 CTGCAC GTGCAG(16/14)
562 CTGCAC
BsrBI 786 CCGCTC CCGCTC(-3/-3)
329 GAGCGG
BstF5I 534 CATCC GGATG(2/0)
687 CATCC
DsaI 413 CCATGG C^C[GA][CT]GG
759 CCGTGG
EarI 43 CTCTTC CTCTTC(1/4)
775 CTCTTC
EcoT14I 413 CCATGG C^C[AT][AT]GG
519 CCTTGG
FokI 534 CATCC GGATG(9/13)
687 CATCC
HaeII 647 GGCGCC [GA]GCGC^[CT]
709 GGCGCT
HphI 570 GGTGA GGTGA(8/7)
285 TCACC
Ksp632I 43 CTCTTC CTCTTC(1/4)
775 CTCTTC
MaeII 352 ACGT A^CGT
698 ACGT
NaeI 163 GCCGGC GCC^GGC
655 GCCGGC
NgoMIV 163 GCCGGC G^CCGGC
655 GCCGGC
RsaI 540 GTAC GT^AC
633 GTAC
SfiI 144 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
804 GGCCTGCCAGGCC
StyI 413 CCATGG C^C[AT][AT]GG
519 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BbvI BcnI BglI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrFI BsrI BssKI BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI TseI TspRI