Restriction map for
pF1KE0432
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KspI MamI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 846 GGTACC G^GTACC
Asp718I 846 GGTACC G^GTACC
AvaI 725 CCCGGG C^[CT]CG[GA]G
BalI 623 TGGCCA TGG^CCA
BanI 846 GGTACC G^G[CT][GA]CC
BbsI 366 GTCTTC GAAGAC(2/6)
BclI 489 TGATCA T^GATCA
BfaI 751 CTAG C^TAG
BpmI 1024 CTGGAG CTGGAG(16/14)
BpuAI 366 GTCTTC GAAGAC(2/6)
BsaAI 273 TACGTA [CT]AC^GT[GA]
BsmBI 30 GAGACG CGTCTC(1/5)
BsmFI 979 GTCCC GGGAC(10/14)
BsmI 451 GAATGC GAATGC(1/-1)
BsoBI 725 CCCGGG C^[CT]CG[GA]G
BsrDI 40 GCAATG GCAATG(2/0)
BstAPI 700 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 943 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1157 CCATGG C^C[GA][CT]GG
EaeI 623 TGGCCA [CT]^GGCC[GA]
EcoT14I 1157 CCATGG C^C[AT][AT]GG
EcoT22I 901 ATGCAT ATGCA^T
FauI 111 GCGGG CCCGC(4/6)
FbaI 489 TGATCA T^GATCA
HindIII 1042 AAGCTT A^AGCTT
HphI 1047 TCACC GGTGA(8/7)
KpnI 846 GGTACC GGTAC^C
MaeI 751 CTAG C^TAG
MluNI 623 TGGCCA TGG^CCA
MscI 623 TGGCCA TGG^CCA
MspA1I 1189 CAGCGG C[AC]G^C[GT]G
NcoI 1157 CCATGG C^CATGG
NsiI 901 ATGCAT ATGCA^T
PleI 703 GAGTC GAGTC(4/5)
PstI 1000 CTGCAG CTGCA^G
SfcI 1000 CTGCAG C^T[GA][CT]AG
SmaI 725 CCCGGG CCC^GGG
SnaBI 273 TACGTA TAC^GTA
SpeI 750 ACTAGT A^CTAGT
SspI 127 AATATT AAT^ATT
StyI 1157 CCATGG C^C[AT][AT]GG
TfiI 1181 GAATC G^A[AT]TC
Tsp45I 23 GTGAC ^GT[GC]AC
XmaCI 725 CCCGGG C^CCGGG
XmaI 725 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 33 ACGTGT A^C[GA][CT]GT
829 ACGTGT
AlwNI 644 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
701 CAGAGTCTG
BglI 721 GCCTCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
730 GCCAAACTGGC
BmyI 516 GGGCAC G[AGT]GC[ACT]^C
1065 GGGCAC
BseRI 102 GAGGAG GAGGAG(10/8)
1204 GAGGAG
BsgI 391 CTGCAC GTGCAG(16/14)
707 CTGCAC
BsiYI 572 CCTGCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
982 CCCGGCATAGG
BslI 572 CCTGCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
982 CCCGGCATAGG
BsmAI 30 GAGAC GTCTC(1/5)
141 GAGAC
Bsp1286I 516 GGGCAC G[AGT]GC[ACT]^C
1065 GGGCAC
BsrBI 148 CCGCTC CCGCTC(-3/-3)
109 GAGCGG
BstF5I 1137 CATCC GGATG(2/0)
1097 GGATG
BstNI 1023 CCTGG CC^[AT]GG
1094 CCTGG
DpnII 490 GATC ^GATC
814 GATC
EcoRII 1023 CCTGG ^CC[AT]GG
1094 CCTGG
FokI 1137 CATCC GGATG(9/13)
1097 GGATG
HapII 726 CCGG C^CGG
983 CCGG
HinfI 703 GAGTC G^A[ACGT]TC
1181 GAATC
HpaII 726 CCGG C^CGG
983 CCGG
MboI 490 GATC ^GATC
814 GATC
MseI 464 TTAA T^TAA
1151 TTAA
MslI 55 CATCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
231 CATTGTGGTG
MspI 726 CCGG C^CGG
983 CCGG
MvaI 1023 CCTGG CC^[AT]GG
1094 CCTGG
NdeII 490 GATC ^GATC
814 GATC
NspI 575 GCATGT [GA]CATG^[CT]
696 ACATGC
Sau3AI 490 GATC ^GATC
814 GATC
Tru9I 464 TTAA T^TAA
1151 TTAA
TspRI 86 CAGTG CA[GC]TG[ACGT][ACGT]^
519 CACTG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BcnI BsaJI BsrI
BssKI Cac8I Cfr13I DdeI EarI
Fnu4HI HaeIII ItaI Ksp632I MaeII
MaeIII MboII MnlI MwoI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI TseI Tsp509I