Restriction map for
pF1KE0979
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AflII Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I AspI AvaI
AviII BbrPI BbvCI BcgI BciVI
BcnI BfrI BsaAI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I ClaI CpoI
DrdI DsaI EagI EclXI Eco52I
EcoO65I EcoT22I FauI FseI FspI
KspI MaeII MluI MroI MvnI
NaeI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI TaqI Tth111I TthHB8I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 78 enzymes) #
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Acc65I 1611 GGTACC G^GTACC
AccI 583 GTAGAC GT^[AC][GT]AC
AflIII 1187 ACATGT A^C[GA][CT]GT
AgeI 59 ACCGGT A^CCGGT
AhdI 1186 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 326 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 727 CCTAAGG CC^T[ACGT]AGG
Aor51HI 848 AGCGCT AGC^GCT
Asp718I 1611 GGTACC G^GTACC
AspEI 1186 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1139 GAGCAC G[AT]GC[AT]^C
AvaII 244 GGACC G^G[AT]CC
AvrII 1114 CCTAGG C^CTAGG
BalI 221 TGGCCA TGG^CCA
BamHI 1294 GGATCC G^GATCC
BanII 903 GAGCCC G[GA]GC[CT]^C
BbeI 560 GGCGCC GGCGC^C
BbsI 1473 GAAGAC GAAGAC(2/6)
BclI 381 TGATCA T^GATCA
BglI 1111 GCCCCTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1650 AGATCT A^GATCT
BlnI 1114 CCTAGG C^CTAGG
BlpI 652 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 652 GCTCAGC GC^T[ACGT]AGC
BpuAI 1473 GAAGAC GAAGAC(2/6)
BsaBI 1651 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 59 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1139 GAGCAC G[AT]GC[AT]^C
BsmAI 1389 GAGAC GTCTC(1/5)
BsmI 500 GCATTC GAATGC(1/-1)
BspHI 920 TCATGA T^CATGA
BspLU11I 1187 ACATGT A^CATGT
BsrFI 59 ACCGGT [GA]^CCGG[CT]
Bsu36I 727 CCTAAGG CC^T[ACGT]AGG
CelII 652 GCTCAGC GC^T[ACGT]AGC
Cfr10I 59 ACCGGT [GA]^CCGG[CT]
DraII 243 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 710 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 221 TGGCCA [CT]^GGCC[GA]
Eam1105I 1186 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 250 GAAGAG CTCTTC(1/4)
Eco47III 848 AGCGCT AGC^GCT
Eco81I 727 CCTAAGG CC^T[ACGT]AGG
EcoNI 1114 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 243 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1232 GATATC GAT^ATC
FbaI 381 TGATCA T^GATCA
HapII 60 CCGG C^CGG
HincII 1422 GTTAAC GT[CT]^[GA]AC
HindII 1422 GTTAAC GT[CT]^[GA]AC
HpaI 1422 GTTAAC GTT^AAC
HpaII 60 CCGG C^CGG
HphI 522 TCACC GGTGA(8/7)
KasI 560 GGCGCC G^GCGCC
KpnI 1611 GGTACC GGTAC^C
Ksp632I 250 GAAGAG CTCTTC(1/4)
MamI 1651 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1181 CAATTG C^AATTG
MluNI 221 TGGCCA TGG^CCA
MscI 221 TGGCCA TGG^CCA
MslI 523 CACCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 305 CAGCTG C[AC]G^C[GT]G
MspI 60 CCGG C^CGG
MunI 1181 CAATTG C^AATTG
NarI 560 GGCGCC GG^CGCC
NheI 1039 GCTAGC G^CTAGC
PflMI 709 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 59 ACCGGT A^CCGGT
PpuMI 243 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 305 CAGCTG CAG^CTG
RcaI 920 TCATGA T^CATGA
SfaNI 1695 GCATC GCATC(5/9)
SfoI 560 GGCGCC GGC^GCC
SmlI 1371 CTTGAG C^T[CT][GA]AG
SphI 1443 GCATGC GCATG^C
SspI 1338 AATATT AAT^ATT
StuI 834 AGGCCT AGG^CCT
Van91I 709 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanI 560 GGCGCC G^G[CT][GA]CC
1611 GGTACC
BbvI 304 GCAGC GCAGC(8/12)
1315 GCTGC
BpmI 1511 CTCCAG CTGGAG(16/14)
409 CTGGAG
BssKI 1129 CCAGG ^CC[ACGT]GG
1513 CCAGG
BstNI 1129 CCAGG CC^[AT]GG
1513 CCAGG
Cac8I 1039 GCTAGC GC[ACGT]^[ACGT]GC
1443 GCATGC
CfoI 561 GCGC GCG^C
849 GCGC
EcoRI 438 GAATTC G^AATTC
1530 GAATTC
EcoRII 1129 CCAGG ^CC[AT]GG
1513 CCAGG
Fnu4HI 304 GCAGC GC^[ACGT]GC
1315 GCTGC
HaeII 560 GGCGCC [GA]GCGC^[CT]
848 AGCGCT
HgaI 7 GCGTC GACGC(5/10)
1098 GCGTC
HhaI 561 GCGC GCG^C
849 GCGC
HinP1I 561 GCGC G^CGC
849 GCGC
ItaI 304 GCAGC GC^[ACGT]GC
1315 GCTGC
MvaI 1129 CCAGG CC^[AT]GG
1513 CCAGG
NspI 1187 ACATGT [GA]CATG^[CT]
1443 GCATGC
PleI 1391 GACTC GAGTC(4/5)
418 GAGTC
PstI 908 CTGCAG CTGCA^G
1196 CTGCAG
ScrFI 1129 CCAGG CC^[ACGT]GG
1513 CCAGG
TseI 304 GCAGC G^C[AT]GC
1315 GCTGC
Tsp45I 56 GTGAC ^GT[GC]AC
1052 GTGAC
XbaI 952 TCTAGA T^CTAGA
1647 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AcyI AfaI AluI AlwI
ApoI BfaI BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BspMI BsrI
BstF5I BstYI Cfr13I DdeI DpnII
DraI EcoT14I FokI HaeIII Hin1I
HindIII HinfI MaeI MaeIII MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I SfcI StyI TfiI Tru9I
Tsp509I TspRI XhoII