Restriction map for
pF1KE0334
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI Asp718I
AspEI AvaI AviII AvrII BbeI
BbvCI BcnI BfrI BglI BglII
BlnI BsaHI BsaI BsiWI BsmBI
BsoBI BspDI BspMI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MspA1I MunI MvnI
NarI NciI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 1763 TCCGGA T^CCGGA
Aor51HI 702 AGCGCT AGC^GCT
AseI 2593 ATTAAT AT^TAAT
AsnI 2593 ATTAAT AT^TAAT
Asp700I 1465 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 444 GTGCTC G[AT]GC[AT]^C
AspI 82 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 438 GGACC G^G[AT]CC
BalI 1547 TGGCCA TGG^CCA
BanI 1641 GGTGCC G^G[CT][GA]CC
BanII 712 GAGCCC G[GA]GC[CT]^C
BbrPI 2500 CACGTG CAC^GTG
BbsI 574 GAAGAC GAAGAC(2/6)
BcgI 252 GCAGAGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 659 GTATCC GTATCC(6/5)
BlpI 949 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 949 GCTCAGC GC^T[ACGT]AGC
BpuAI 574 GAAGAC GAAGAC(2/6)
BsaAI 2500 CACGTG [CT]AC^GT[GA]
BsaWI 1763 TCCGGA [AT]^CCGG[AT]
BseAI 1763 TCCGGA T^CCGGA
BsiEI 86 CGGTCG CG[GA][CT]^CG
BsiHKAI 444 GTGCTC G[AT]GC[AT]^C
BsmI 794 GCATTC GAATGC(1/-1)
BspEI 1763 TCCGGA T^CCGGA
BspHI 426 TCATGA T^CATGA
BssSI 260 CTCGTG CACGAG(-5/-1)
BstAPI 166 GCATGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 949 GCTCAGC GC^T[ACGT]AGC
CfoI 703 GCGC GCG^C
DrdI 2496 GACCCACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 826 GAAGAG CTCTTC(1/4)
Eco47III 702 AGCGCT AGC^GCT
EcoRV 537 GATATC GAT^ATC
FauI 6 GCGGG CCCGC(4/6)
FseI 9 GGCCGGCC GGCCGG^CC
HaeII 702 AGCGCT [GA]GCGC^[CT]
HhaI 703 GCGC GCG^C
HinP1I 703 GCGC G^CGC
HincII 763 GTTGAC GT[CT]^[GA]AC
HindII 763 GTTGAC GT[CT]^[GA]AC
HindIII 1478 AAGCTT A^AGCTT
Ksp632I 826 GAAGAG CTCTTC(1/4)
MluNI 1547 TGGCCA TGG^CCA
MroI 1763 TCCGGA T^CCGGA
MscI 1547 TGGCCA TGG^CCA
NaeI 10 GCCGGC GCC^GGC
NdeI 734 CATATG CA^TATG
NgoMIV 10 GCCGGC G^CCGGC
PflFI 82 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1956 CCACGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2500 CACGTG CAC^GTG
PmlI 2500 CACGTG CAC^GTG
PshBI 2593 ATTAAT AT^TAAT
PstI 2535 CTGCAG CTGCA^G
RcaI 426 TCATGA T^CATGA
SmlI 447 CTCAAG C^T[CT][GA]AG
Tth111I 82 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1956 CCACGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1465 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BamHI 741 GGATCC G^GATCC
1505 GGATCC
BclI 423 TGATCA T^GATCA
2361 TGATCA
BmyI 444 GTGCTC G[AGT]GC[ACT]^C
712 GAGCCC
BpmI 973 CTGGAG CTGGAG(16/14)
1921 CTGGAG
BsaBI 1960 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2242 GATCCTCATC
BseRI 1423 GAGGAG GAGGAG(10/8)
2195 GAGGAG
Bsp1286I 444 GTGCTC G[AGT]GC[ACT]^C
712 GAGCCC
BspLU11I 693 ACATGT A^CATGT
1226 ACATGT
BsrBI 1880 CCGCTC CCGCTC(-3/-3)
4 GAGCGG
BsrDI 1934 CATTGC GCAATG(2/0)
2269 GCAATG
BsrFI 10 GCCGGC [GA]^CCGG[CT]
320 ACCGGC
BstXI 149 CCAGAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1550 CCAAGCAAATGG
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
320 ACCGGC
DraII 2489 AGGCCCT [GA]G^G[ACGT]CC[CT]
2561 AGGCCCT
EaeI 1547 TGGCCA [CT]^GGCC[GA]
2607 CGGCCA
EcoO109I 2489 AGGCCCT [GA]G^G[ACGT]CC[CT]
2561 AGGCCCT
EcoT22I 164 ATGCAT ATGCA^T
731 ATGCAT
FbaI 423 TGATCA T^GATCA
2361 TGATCA
HphI 709 GGTGA GGTGA(8/7)
1968 TCACC
MamI 1960 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2242 GATCCTCATC
NcoI 528 CCATGG C^CATGG
1544 CCATGG
NsiI 164 ATGCAT ATGCA^T
731 ATGCAT
TaqI 89 TCGA T^CGA
1001 TCGA
TthHB8I 89 TCGA T^CGA
1001 TCGA
XcmI 146 CCACCAGAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1103 CCATTTTCATAGTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI BbvI BfaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII