Restriction map for
pF1KE0311
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BciVI BfrI BglI BlnI BsaAI
BsaI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI HinP1I KasI KpnI KspI
MluI MroI MvnI MwoI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I StuI SwaI TseI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 3318 GTCTAC GT^[AC][GT]AC
AciI 777 CCGC CCGC(-3/-1)
AlwNI 3089 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 38 CCTCAGG CC^T[ACGT]AGG
AseI 1825 ATTAAT AT^TAAT
AsnI 1825 ATTAAT AT^TAAT
AspHI 3537 GTGCTC G[AT]GC[AT]^C
BalI 2370 TGGCCA TGG^CCA
BcgI 1118 GCAAAATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2919 TGATCA T^GATCA
BlpI 2686 GCTGAGC GC^T[ACGT]AGC
BpmI 3266 CTCCAG CTGGAG(16/14)
Bpu1102I 2686 GCTGAGC GC^T[ACGT]AGC
BsaBI 3091 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1331 GTGCAG GTGCAG(16/14)
BsiHKAI 3537 GTGCTC G[AT]GC[AT]^C
BsmFI 1753 GGGAC GGGAC(10/14)
BsmI 2857 GAATGC GAATGC(1/-1)
Bsp1407I 2496 TGTACA T^GTACA
BspHI 338 TCATGA T^CATGA
BsrGI 2496 TGTACA T^GTACA
BstF5I 1317 GGATG GGATG(2/0)
BstXI 765 CCAGTCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 38 CCTCAGG CC^T[ACGT]AGG
CelII 2686 GCTGAGC GC^T[ACGT]AGC
DrdI 3225 GACGTCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 567 CTCTTC CTCTTC(1/4)
Eco81I 38 CCTCAGG CC^T[ACGT]AGG
EcoT14I 3530 CCTTGG C^C[AT][AT]GG
FbaI 2919 TGATCA T^GATCA
Fnu4HI 776 GCCGC GC^[ACGT]GC
FokI 1317 GGATG GGATG(9/13)
HgaI 456 GCGTC GACGC(5/10)
HpaI 3024 GTTAAC GTT^AAC
ItaI 776 GCCGC GC^[ACGT]GC
Ksp632I 567 CTCTTC CTCTTC(1/4)
MamI 3091 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1063 CAATTG C^AATTG
MluNI 2370 TGGCCA TGG^CCA
MscI 2370 TGGCCA TGG^CCA
MunI 1063 CAATTG C^AATTG
NheI 415 GCTAGC G^CTAGC
PshBI 1825 ATTAAT AT^TAAT
PstI 857 CTGCAG CTGCA^G
RcaI 338 TCATGA T^CATGA
SpeI 3079 ACTAGT A^CTAGT
SspBI 2496 TGTACA T^GTACA
SspI 535 AATATT AAT^ATT
StyI 3530 CCTTGG C^C[AT][AT]GG
XbaI 1600 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1687 GACGTC GACGT^C
3225 GACGTC
AcyI 1687 GACGTC G[GA]^CG[CT]C
3225 GACGTC
AflIII 1883 ACATGT A^C[GA][CT]GT
2793 ACATGT
Asp700I 1185 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2449 GAAATAATTC
AvaII 1754 GGACC G^G[AT]CC
2051 GGACC
BcnI 1342 CCCGG CC^[GC]GG
2263 CCCGG
BmyI 6 GTGCCC G[AGT]GC[ACT]^C
3537 GTGCTC
BsaHI 1687 GACGTC G[GA]^CG[CT]C
3225 GACGTC
BsaJI 2262 CCCCGG C^C[ACGT][ACGT]GG
3530 CCTTGG
BsaWI 1850 ACCGGA [AT]^CCGG[AT]
3605 ACCGGA
Bsp1286I 6 GTGCCC G[AGT]GC[ACT]^C
3537 GTGCTC
BspLU11I 1883 ACATGT A^CATGT
2793 ACATGT
BspMI 2298 ACCTGC ACCTGC(4/8)
2385 ACCTGC
Cfr13I 1754 GGACC G^G[ACGT]CC
2051 GGACC
DraII 1753 GGGACCC [GA]G^G[ACGT]CC[CT]
2050 AGGACCC
EaeI 774 TGGCCG [CT]^GGCC[GA]
2370 TGGCCA
EcoO109I 1753 GGGACCC [GA]G^G[ACGT]CC[CT]
2050 AGGACCC
EcoRI 210 GAATTC G^AATTC
1569 GAATTC
Hin1I 1687 GACGTC G[GA]^CG[CT]C
3225 GACGTC
MspA1I 1366 CAGCTG C[AC]G^C[GT]G
1387 CAGCTG
NciI 1342 CCCGG CC^[GC]GG
2263 CCCGG
NspI 1883 ACATGT [GA]CATG^[CT]
2793 ACATGT
PleI 3126 GACTC GAGTC(4/5)
1003 GAGTC
PpuMI 1753 GGGACCC [GA]G^G[AT]CC[CT]
2050 AGGACCC
PvuII 1366 CAGCTG CAG^CTG
1387 CAGCTG
Sau96I 1754 GGACC G^G[ACGT]CC
2051 GGACC
TaqI 216 TCGA T^CGA
1268 TCGA
TthHB8I 216 TCGA T^CGA
1268 TCGA
XmnI 1185 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2449 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BfaI BglII BpuAI BsiYI
BslI BsmAI BsrI BssKI BstNI
BstYI Cac8I DdeI DpnII DraI
EcoRII HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI SfcI TfiI Tru9I
Tsp45I Tsp509I TspRI XhoII