Restriction map for
pF1KE0278
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MroI MseI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 535 GTCTAC GT^[AC][GT]AC
AluI 180 AGCT AG^CT
AocI 583 CCTGAGG CC^T[ACGT]AGG
AvaII 378 GGACC G^G[AT]CC
BclI 387 TGATCA T^GATCA
BfaI 119 CTAG C^TAG
BpmI 111 CTCCAG CTGGAG(16/14)
BspHI 491 TCATGA T^CATGA
BspMI 595 ACCTGC ACCTGC(4/8)
BsrI 311 ACTGG ACTGG(1/-1)
BstEII 591 GGTCACC G^GT[ACGT]ACC
BstPI 591 GGTCACC G^GT[ACGT]ACC
Bsu36I 583 CCTGAGG CC^T[ACGT]AGG
DpnII 388 GATC ^GATC
Eco81I 583 CCTGAGG CC^T[ACGT]AGG
EcoO65I 591 GGTCACC G^GT[ACGT]ACC
EcoT22I 459 ATGCAT ATGCA^T
FauI 622 CCCGC CCCGC(4/6)
FbaI 387 TGATCA T^GATCA
MaeI 119 CTAG C^TAG
MaeII 502 ACGT A^CGT
MboI 388 GATC ^GATC
NcoI 209 CCATGG C^CATGG
NdeII 388 GATC ^GATC
NsiI 459 ATGCAT ATGCA^T
RcaI 491 TCATGA T^CATGA
Sau3AI 388 GATC ^GATC
TaqI 398 TCGA T^CGA
TthHB8I 398 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 577 CCGC CCGC(-3/-1)
623 CCGC
AfaI 504 GTAC GT^AC
516 GTAC
ApaI 75 GGGCCC GGGCC^C
546 GGGCCC
Asp700I 132 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
135 GAAGTTCTTC
BalI 149 TGGCCA TGG^CCA
288 TGGCCA
BanII 75 GGGCCC G[GA]GC[CT]^C
546 GGGCCC
BmyI 75 GGGCCC G[AGT]GC[ACT]^C
546 GGGCCC
Bsp1286I 75 GGGCCC G[AGT]GC[ACT]^C
546 GGGCCC
CfoI 236 GCGC GCG^C
616 GCGC
DdeI 410 CTGAG C^T[ACGT]AG
584 CTGAG
EaeI 149 TGGCCA [CT]^GGCC[GA]
288 TGGCCA
HaeII 235 AGCGCC [GA]GCGC^[CT]
615 AGCGCC
HhaI 236 GCGC GCG^C
616 GCGC
HinP1I 236 GCGC G^CGC
616 GCGC
MboII 132 GAAGA GAAGA(8/7)
140 TCTTC
MluNI 149 TGGCCA TGG^CCA
288 TGGCCA
MscI 149 TGGCCA TGG^CCA
288 TGGCCA
MslI 367 CACCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
458 CATGCATGTG
NspI 457 ACATGC [GA]CATG^[CT]
461 GCATGT
RsaI 504 GTAC GT^AC
516 GTAC
SfaNI 56 GATGC GCATC(5/9)
566 GCATC
TspRI 408 CACTG CA[GC]TG[ACGT][ACGT]^
423 CAGTG
XcmI 113 CCAGGTCTAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
296 CCAAGGATGAGGTGG
XmnI 132 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
135 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
BbvI BsaJI BseRI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HphI ItaI
MaeIII MnlI MvaI MwoI NlaIII
NlaIV PstI Sau96I ScrFI SfcI
StyI TseI Tsp45I