Restriction map for
pF1KE0273
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# NO CUT ENZYMES ( 198 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DdeI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HapII
HincII HindII HindIII HpaI HpaII
KpnI Ksp632I MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MspI MunI NaeI
NciI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI Sau3AI Sau96I SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I TseI Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AfaI 264 GTAC GT^AC
Asp700I 177 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 43 GGCGCC G^G[CT][GA]CC
BbeI 43 GGCGCC GGCGC^C
BfaI 31 CTAG C^TAG
BglI 119 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmFI 282 GGGAC GGGAC(10/14)
BstXI 251 CCATGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 6 TGGCCG [CT]^GGCC[GA]
FauI 97 CCCGC CCCGC(4/6)
HinfI 86 GAATC G^A[ACGT]TC
KasI 43 GGCGCC G^GCGCC
MaeI 31 CTAG C^TAG
MaeII 257 ACGT A^CGT
NarI 43 GGCGCC GG^CGCC
NcoI 251 CCATGG C^CATGG
NlaIII 252 CATG CATG^
NlaIV 43 GGCGCC GG[ACGT]^[ACGT]CC
RsaI 264 GTAC GT^AC
SfaNI 205 GATGC GCATC(5/9)
SfoI 43 GGCGCC GGC^GCC
SmlI 271 CTCAAG C^T[CT][GA]AG
TfiI 86 GAATC G^A[AT]TC
XmnI 177 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcyI 43 GGCGCC G[GA]^CG[CT]C
127 GGCGTC
BsaHI 43 GGCGCC G[GA]^CG[CT]C
127 GGCGTC
BsiYI 9 CCGCGGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
105 CCGCCGCCTGG
BslI 9 CCGCGGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
105 CCGCCGCCTGG
Cac8I 96 GCCCGC GC[ACGT]^[ACGT]GC
115 GCTCGC
EcoT14I 81 CCAAGG C^C[AT][AT]GG
251 CCATGG
HaeII 43 GGCGCC [GA]GCGC^[CT]
275 AGCGCC
HaeIII 7 GGCC GG^CC
145 GGCC
Hin1I 43 GGCGCC G[GA]^CG[CT]C
127 GGCGTC
KspI 9 CCGCGG CCGC^GG
120 CCGCGG
MnlI 147 CCTC CCTC(7/6)
157 GAGG
MslI 246 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
252 CATGGACGTG
MspA1I 9 CCGCGG C[AC]G^C[GT]G
120 CCGCGG
SacII 9 CCGCGG CCGC^GG
120 CCGCGG
StyI 81 CCAAGG C^C[AT][AT]GG
251 CCATGG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AccII AciI BsaJI BssKI BstNI
BstUI CfoI DsaI EcoRII Fnu4HI
HgaI HhaI HinP1I HphI ItaI
MaeIII MvaI MvnI MwoI ScrFI
Tsp45I