Restriction map for
pF1KE0272
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BspDI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DpnII DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I MamI MboI
MboII MfeI MflI MluI MseI
MunI NaeI NarI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 242 TCCGGA T^CCGGA
ApaI 585 GGGCCC GGGCC^C
BalI 488 TGGCCA TGG^CCA
BanI 619 GGTGCC G^G[CT][GA]CC
BbrPI 163 CACGTG CAC^GTG
BfaI 556 CTAG C^TAG
BsaAI 163 CACGTG [CT]AC^GT[GA]
BsaI 559 GAGACC GGTCTC(1/5)
BseAI 242 TCCGGA T^CCGGA
BseRI 425 CTCCTC GAGGAG(10/8)
BsgI 361 GTGCAG GTGCAG(16/14)
BsmAI 559 GAGAC GTCTC(1/5)
BspEI 242 TCCGGA T^CCGGA
BspMI 81 GCAGGT ACCTGC(4/8)
CfoI 88 GCGC GCG^C
DrdI 295 GACGTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 87 GGCGCT [GA]GCGC^[CT]
HhaI 88 GCGC GCG^C
HinP1I 88 GCGC G^CGC
KspI 24 CCGCGG CCGC^GG
MaeI 556 CTAG C^TAG
MluNI 488 TGGCCA TGG^CCA
MroI 242 TCCGGA T^CCGGA
MscI 488 TGGCCA TGG^CCA
NcoI 419 CCATGG C^CATGG
NspI 377 GCATGC [GA]CATG^[CT]
PmaCI 163 CACGTG CAC^GTG
PmlI 163 CACGTG CAC^GTG
SacII 24 CCGCGG CCGC^GG
SmaI 5 CCCGGG CCC^GGG
SphI 377 GCATGC GCATG^C
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 25 CGCG CG^CG
99 CGCG
AvaI 5 CCCGGG C^[CT]CG[GA]G
549 CTCGGG
BanII 169 GGGCTC G[GA]GC[CT]^C
585 GGGCCC
BpmI 566 CTCCAG CTGGAG(16/14)
147 CTGGAG
BsaWI 227 TCCGGT [AT]^CCGG[AT]
242 TCCGGA
BsoBI 5 CCCGGG C^[CT]CG[GA]G
549 CTCGGG
Bsp1407I 330 TGTACA T^GTACA
443 TGTACA
BsrGI 330 TGTACA T^GTACA
443 TGTACA
BstF5I 174 CATCC GGATG(2/0)
306 CATCC
BstUI 25 CGCG CG^CG
99 CGCG
DraII 544 GGGGCCT [GA]G^G[ACGT]CC[CT]
585 GGGCCCC
DsaI 24 CCGCGG C^C[GA][CT]GG
419 CCATGG
EaeI 17 TGGCCG [CT]^GGCC[GA]
488 TGGCCA
EcoO109I 544 GGGGCCT [GA]G^G[ACGT]CC[CT]
585 GGGCCCC
EcoT14I 419 CCATGG C^C[AT][AT]GG
540 CCTTGG
FokI 174 CATCC GGATG(9/13)
306 CATCC
HinfI 415 GACTC G^A[ACGT]TC
580 GAGTC
MaeII 164 ACGT A^CGT
296 ACGT
MslI 306 CATCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
564 CACTCCAGTG
MvnI 25 CGCG CG^CG
99 CGCG
NlaIII 378 CATG CATG^
420 CATG
PleI 415 GACTC GAGTC(4/5)
580 GAGTC
PvuII 108 CAGCTG CAG^CTG
215 CAGCTG
SfcI 351 CTACAG C^T[GA][CT]AG
435 CTACAG
SspBI 330 TGTACA T^GTACA
443 TGTACA
StyI 419 CCATGG C^C[AT][AT]GG
540 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrBI BsrI BssKI BstNI
Cac8I Cfr13I EcoRII FauI Fnu4HI
HaeIII HapII HpaII ItaI MaeIII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV RsaI Sau96I ScrFI
TseI Tsp45I TspRI