Restriction map for
pF1KE0266
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AlwI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbrPI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseRI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MseI MunI NciI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 673 GACGTC GACGT^C
AccIII 8 TCCGGA T^CCGGA
AflIII 716 ACGTGT A^C[GA][CT]GT
Aor51HI 1238 AGCGCT AGC^GCT
AspI 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 418 GGCGCC GGCGC^C
BbsI 838 GTCTTC GAAGAC(2/6)
BcgI 1159 CGATGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 242 GGATAC GTATCC(6/5)
BpuAI 838 GTCTTC GAAGAC(2/6)
BsaI 135 GAGACC GGTCTC(1/5)
BsaWI 8 TCCGGA [AT]^CCGG[AT]
BseAI 8 TCCGGA T^CCGGA
BsmAI 135 GAGAC GTCTC(1/5)
BspEI 8 TCCGGA T^CCGGA
BsrDI 1203 CATTGC GCAATG(2/0)
BstAPI 452 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1307 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 195 GATC ^GATC
Eco47III 1238 AGCGCT AGC^GCT
KasI 418 GGCGCC G^GCGCC
MboI 195 GATC ^GATC
MroI 8 TCCGGA T^CCGGA
MspA1I 1136 CAGCGG C[AC]G^C[GT]G
NarI 418 GGCGCC GG^CGCC
NdeII 195 GATC ^GATC
NotI 552 GCGGCCGC GC^GGCCGC
PflFI 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 278 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 918 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 195 GATC ^GATC
ScaI 899 AGTACT AGT^ACT
SexAI 218 ACCAGGT A^CC[AT]GGT
SfoI 418 GGCGCC GGC^GCC
SmlI 91 CTCAAG C^T[CT][GA]AG
Tth111I 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AfaI 349 GTAC GT^AC
900 GTAC
ApaI 1010 GGGCCC GGGCC^C
1140 GGGCCC
AspHI 637 GTGCTC G[AT]GC[AT]^C
742 GTGCTC
BalI 908 TGGCCA TGG^CCA
1292 TGGCCA
BanI 418 GGCGCC G^G[CT][GA]CC
536 GGCACC
BbvCI 21 CCTCAGC CCTCAGC(-5/-2)
73 GCTGAGG
BfaI 1007 CTAG C^TAG
1100 CTAG
BpmI 377 CTCCAG CTGGAG(16/14)
175 CTGGAG
BsaAI 715 TACGTG [CT]AC^GT[GA]
1192 CACGTA
BsgI 1186 CTGCAC GTGCAG(16/14)
1339 GTGCAG
BsiEI 553 CGGCCG CG[GA][CT]^CG
872 CGGCCG
BsiHKAI 637 GTGCTC G[AT]GC[AT]^C
742 GTGCTC
BspHI 956 TCATGA T^CATGA
1319 TCATGA
BsrBI 763 CCGCTC CCGCTC(-3/-3)
886 CCGCTC
BssSI 1150 CACGAG CACGAG(-5/-1)
456 CTCGTG
DsaI 920 CCATGG C^C[GA][CT]GG
1307 CCATGG
EagI 553 CGGCCG C^GGCCG
872 CGGCCG
EclXI 553 CGGCCG C^GGCCG
872 CGGCCG
Eco52I 553 CGGCCG C^GGCCG
872 CGGCCG
EcoNI 1001 CCTGGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1114 CCTGAAGGAGG
EcoT14I 920 CCATGG C^C[AT][AT]GG
1307 CCATGG
MaeI 1007 CTAG C^TAG
1100 CTAG
MluNI 908 TGGCCA TGG^CCA
1292 TGGCCA
MscI 908 TGGCCA TGG^CCA
1292 TGGCCA
NcoI 920 CCATGG C^CATGG
1307 CCATGG
NspI 617 GCATGC [GA]CATG^[CT]
863 GCATGC
PstI 56 CTGCAG CTGCA^G
566 CTGCAG
RcaI 956 TCATGA T^CATGA
1319 TCATGA
RsaI 349 GTAC GT^AC
900 GTAC
SfcI 56 CTGCAG C^T[GA][CT]AG
566 CTGCAG
SphI 617 GCATGC GCATG^C
863 GCATGC
StyI 920 CCATGG C^C[AT][AT]GG
1307 CCATGG
TaqI 200 TCGA T^CGA
488 TCGA
TthHB8I 200 TCGA T^CGA
488 TCGA
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaII BanII BbvI BglI BmyI
BsaHI BsaJI BsiYI BslI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MnlI
MslI MspI MvaI MvnI MwoI
NaeI NgoMIV NlaIII NlaIV PleI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI XcmI