##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MluI        MluNI       MscI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tsp45I      Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII         32  TCCGGA               T^CCGGA
AlwI          309  GGATC                GGATC(4/5)
ApaI          106  GGGCCC               GGGCC^C
Asp700I        63  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         244  GTGCTC               G[AT]GC[AT]^C
BglI          160  GCCCTACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          404  CTGGAG               CTGGAG(16/14)
BsaWI          32  TCCGGA               [AT]^CCGG[AT]
BseAI          32  TCCGGA               T^CCGGA
BseRI         407  GAGGAG               GAGGAG(10/8)
BsiHKAI       244  GTGCTC               G[AT]GC[AT]^C
BspEI          32  TCCGGA               T^CCGGA
BsrDI         378  GCAATG               GCAATG(2/0)
BstAPI        394  GCACTTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I         48  GGATG                GGATG(2/0)
BstNI          99  CCAGG                CC^[AT]GG
BstYI         309  GGATCT               [GA]^GATC[CT]
DpnII         310  GATC                 ^GATC
DraI          256  TTTAAA               TTT^AAA
EaeI           96  CGGCCA               [CT]^GGCC[GA]
EcoRII         99  CCAGG                ^CC[AT]GG
FauI           26  CCCGC                CCCGC(4/6)
FokI           48  GGATG                GGATG(9/13)
MaeII          44  ACGT                 A^CGT
MaeIII        372  GTTAC                ^GT[ACGT]AC
MboI          310  GATC                 ^GATC
MflI          309  GGATCT               [GA]^GATC[CT]
MroI           32  TCCGGA               T^CCGGA
MseI          257  TTAA                 T^TAA
MspA1I        155  CAGCTG               C[AC]G^C[GT]G
MvaI           99  CCAGG                CC^[AT]GG
NdeII         310  GATC                 ^GATC
PvuII         155  CAGCTG               CAG^CTG
Sau3AI        310  GATC                 ^GATC
SfcI          163  CTACAG               C^T[GA][CT]AG
TaqI            4  TCGA                 T^CGA
Tru9I         257  TTAA                 T^TAA
TthHB8I         4  TCGA                 T^CGA
XhoII         309  GGATCT               [GA]^GATC[CT]
XmnI           63  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI           27  CCGC                 CCGC(-3/-1)
              138  GCGG                
AvaII         216  GGACC                G^G[AT]CC
              416  GGTCC               
BanII         106  GGGCCC               G[GA]GC[CT]^C
              345  GAGCCC              
BcnI          104  CCGGG                CC^[GC]GG
              219  CCGGG               
HinfI         127  GACTC                G^A[ACGT]TC
              201  GAGTC               
MwoI          160  GCCCTACAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              394  GCACTTGCTGC         
NciI          104  CCGGG                CC^[GC]GG
              219  CCGGG               
NlaIII        150  CATG                 CATG^
              385  CATG                
NlaIV         106  GGGCCC               GG[ACGT]^[ACGT]CC
              415  GGGTCC              
PleI          127  GACTC                GAGTC(4/5)
              201  GAGTC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        BbvI        BmyI        BsiYI       BslI        
Bsp1286I    BssKI       Cac8I       Cfr13I      Fnu4HI      
HaeIII      HapII       HpaII       ItaI        MboII       
MnlI        MspI        Sau96I      ScrFI       TseI        
TspRI