Restriction map for
pF1KE0257
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AocI 541 CCTGAGG CC^T[ACGT]AGG
Aor51HI 424 AGCGCT AGC^GCT
AspHI 364 GTGCTC G[AT]GC[AT]^C
BbsI 486 GAAGAC GAAGAC(2/6)
BclI 167 TGATCA T^GATCA
BfaI 722 CTAG C^TAG
BglII 276 AGATCT A^GATCT
BlpI 996 GCTGAGC GC^T[ACGT]AGC
BpmI 122 CTCCAG CTGGAG(16/14)
Bpu1102I 996 GCTGAGC GC^T[ACGT]AGC
BpuAI 486 GAAGAC GAAGAC(2/6)
BsiHKAI 364 GTGCTC G[AT]GC[AT]^C
BsmAI 622 GTCTC GTCTC(1/5)
Bst1107I 562 GTATAC GTA^TAC
BstZ17I 562 GTATAC GTA^TAC
Bsu36I 541 CCTGAGG CC^T[ACGT]AGG
CelII 996 GCTGAGC GC^T[ACGT]AGC
CfoI 425 GCGC GCG^C
DraI 1021 TTTAAA TTT^AAA
DraII 724 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1161 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 424 AGCGCT AGC^GCT
Eco81I 541 CCTGAGG CC^T[ACGT]AGG
EcoO109I 724 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 186 CCTTGG C^C[AT][AT]GG
EcoT22I 919 ATGCAT ATGCA^T
FauI 110 GCGGG CCCGC(4/6)
FbaI 167 TGATCA T^GATCA
HaeII 424 AGCGCT [GA]GCGC^[CT]
HaeIII 1055 GGCC GG^CC
HgaI 509 GCGTC GACGC(5/10)
HhaI 425 GCGC GCG^C
HinP1I 425 GCGC G^CGC
HindIII 1005 AAGCTT A^AGCTT
MaeI 722 CTAG C^TAG
MaeIII 1150 GTGAC ^GT[ACGT]AC
NsiI 919 ATGCAT ATGCA^T
PpuMI 724 AGGACCC [GA]G^G[AT]CC[CT]
SmaI 1113 CCCGGG CCC^GGG
SmlI 457 CTTGAG C^T[CT][GA]AG
SspI 247 AATATT AAT^ATT
StyI 186 CCTTGG C^C[AT][AT]GG
TaqI 573 TCGA T^CGA
Tsp45I 1150 GTGAC ^GT[GC]AC
TthHB8I 573 TCGA T^CGA
XcmI 809 CCAGAACGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1113 CCCGGG C^CCGGG
XmaI 1113 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 608 GTAC GT^AC
870 GTAC
AlwI 577 GATCC GGATC(4/5)
515 GGATC
AvaI 944 CTCGGG C^[CT]CG[GA]G
1113 CCCGGG
AvaII 725 GGACC G^G[AT]CC
1110 GGTCC
BbvI 12 GCAGC GCAGC(8/12)
506 GCTGC
BcnI 1113 CCCGG CC^[GC]GG
1114 CCGGG
BmyI 364 GTGCTC G[AGT]GC[ACT]^C
1116 GGGCAC
BsiYI 842 CCCTCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1114 CCGGGCACTGG
BslI 842 CCCTCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1114 CCGGGCACTGG
BsmFI 113 GGGAC GGGAC(10/14)
1111 GTCCC
BsoBI 944 CTCGGG C^[CT]CG[GA]G
1113 CCCGGG
Bsp1286I 364 GTGCTC G[AGT]GC[ACT]^C
1116 GGGCAC
BsrBI 108 GAGCGG CCGCTC(-3/-3)
1051 GAGCGG
BstAPI 136 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
962 GCAATTCTTGC
BstYI 276 AGATCT [GA]^GATC[CT]
576 AGATCC
Cac8I 502 GCTGGC GC[ACGT]^[ACGT]GC
1179 GCAGGC
Cfr13I 725 GGACC G^G[ACGT]CC
1110 GGTCC
HapII 378 CCGG C^CGG
1114 CCGG
HpaII 378 CCGG C^CGG
1114 CCGG
MboII 347 GAAGA GAAGA(8/7)
486 GAAGA
MflI 276 AGATCT [GA]^GATC[CT]
576 AGATCC
MslI 171 CATTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1128 CATACAGATG
MspI 378 CCGG C^CGG
1114 CCGG
NciI 1113 CCCGG CC^[GC]GG
1114 CCGGG
NlaIV 725 GGACCC GG[ACGT]^[ACGT]CC
1110 GGTCCC
RsaI 608 GTAC GT^AC
870 GTAC
Sau96I 725 GGACC G^G[ACGT]CC
1110 GGTCC
SfaNI 310 GATGC GCATC(5/9)
182 GCATC
TseI 12 GCAGC G^C[AT]GC
506 GCTGC
XhoII 276 AGATCT [GA]^GATC[CT]
576 AGATCC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccI AciI AluI BsaJI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HinfI HphI
ItaI MboI MnlI MseI MvaI
MwoI NdeII NlaIII Sau3AI ScrFI
TfiI Tru9I Tsp509I TspRI