Restriction map for
pF1KE0253
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI TfiI Tru9I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 581 GGTACC G^GTACC
AlwNI 243 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 630 CCTGAGG CC^T[ACGT]AGG
Asp718I 581 GGTACC G^GTACC
AspHI 1015 GAGCTC G[AT]GC[AT]^C
AspI 80 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 288 TGGCCA TGG^CCA
BanI 581 GGTACC G^G[CT][GA]CC
BbsI 700 GTCTTC GAAGAC(2/6)
BciVI 1059 GGATAC GTATCC(6/5)
BclI 773 TGATCA T^GATCA
BpuAI 700 GTCTTC GAAGAC(2/6)
BsaAI 235 TACGTG [CT]AC^GT[GA]
BsaWI 104 ACCGGA [AT]^CCGG[AT]
BsgI 472 GTGCAG GTGCAG(16/14)
BsiHKAI 1015 GAGCTC G[AT]GC[AT]^C
BsmI 157 GCATTC GAATGC(1/-1)
BspMI 365 ACCTGC ACCTGC(4/8)
BsrBI 577 GAGCGG CCGCTC(-3/-3)
BsrFI 369 GCCGGT [GA]^CCGG[CT]
BssSI 469 CTCGTG CACGAG(-5/-1)
BstAPI 474 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 488 AGATCC [GA]^GATC[CT]
Bsu36I 630 CCTGAGG CC^T[ACGT]AGG
Cfr10I 369 GCCGGT [GA]^CCGG[CT]
EaeI 288 TGGCCA [CT]^GGCC[GA]
EarI 498 GAAGAG CTCTTC(1/4)
Eco81I 630 CCTGAGG CC^T[ACGT]AGG
EcoT14I 678 CCTTGG C^C[AT][AT]GG
FauI 68 CCCGC CCCGC(4/6)
FbaI 773 TGATCA T^GATCA
HgaI 84 GCGTC GACGC(5/10)
HinfI 256 GACTC G^A[ACGT]TC
KpnI 581 GGTACC GGTAC^C
Ksp632I 498 GAAGAG CTCTTC(1/4)
MaeII 236 ACGT A^CGT
MflI 488 AGATCC [GA]^GATC[CT]
MluNI 288 TGGCCA TGG^CCA
MscI 288 TGGCCA TGG^CCA
PflFI 80 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 256 GACTC GAGTC(4/5)
PpuMI 63 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 756 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 20 CTGCAG CTGCA^G
SacI 1015 GAGCTC GAGCT^C
SfcI 20 CTGCAG C^T[GA][CT]AG
SspI 1081 AATATT AAT^ATT
StyI 678 CCTTGG C^C[AT][AT]GG
Tsp45I 3 GTCAC ^GT[GC]AC
Tth111I 80 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 488 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 562 GAATTC [GA]^AATT[CT]
715 GAATTC
AlwI 489 GATCC GGATC(4/5)
984 GATCC
ApoI 562 GAATTC [GA]^AATT[CT]
715 GAATTC
AvaII 64 GGACC G^G[AT]CC
102 GGACC
BanII 129 GGGCTC G[GA]GC[CT]^C
1015 GAGCTC
BbvCI 405 CCTCAGC CCTCAGC(-5/-2)
939 CCTCAGC
BmyI 129 GGGCTC G[AGT]GC[ACT]^C
1015 GAGCTC
BpmI 822 CTCCAG CTGGAG(16/14)
532 CTGGAG
BsaI 754 GAGACC GGTCTC(1/5)
182 GGTCTC
BseRI 174 CTCCTC GAGGAG(10/8)
937 CTCCTC
BsiYI 98 CCCTGGACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCATAAGCAGG
BslI 98 CCCTGGACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCATAAGCAGG
BsmAI 754 GAGAC GTCTC(1/5)
183 GTCTC
Bsp1286I 129 GGGCTC G[AGT]GC[ACT]^C
1015 GAGCTC
DraII 63 GGGACCC [GA]G^G[ACGT]CC[CT]
323 AGGCCCT
DrdI 103 GACCGGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
789 GACATCACTGTC
EcoO109I 63 GGGACCC [GA]G^G[ACGT]CC[CT]
323 AGGCCCT
EcoRI 562 GAATTC G^AATTC
715 GAATTC
MaeIII 3 GTCAC ^GT[ACGT]AC
209 GTAAC
MslI 691 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
875 CACGGCTATG
Tsp509I 563 AATT ^AATT
716 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BsaJI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI TseI TspRI