##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboI        MboII       MfeI        MflI        MluI        
MroI        MseI        MslI        MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   20 enzymes) #
###################################

ApaI          118  GGGCCC               GGGCC^C
BalI          243  TGGCCA               TGG^CCA
BsaI          167  GGTCTC               GGTCTC(1/5)
BsmAI         168  GTCTC                GTCTC(1/5)
BspMI         164  GCAGGT               ACCTGC(4/8)
BstAPI        134  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI          132  GCGC                 GCG^C
DdeI          145  CTGAG                C^T[ACGT]AG
EaeI          243  TGGCCA               [CT]^GGCC[GA]
EcoNI         200  CCTGTGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI          132  GCGC                 GCG^C
HinP1I        132  GCGC                 G^CGC
HinfI         147  GAGTC                G^A[ACGT]TC
MluNI         243  TGGCCA               TGG^CCA
MscI          243  TGGCCA               TGG^CCA
NlaIV         118  GGGCCC               GG[ACGT]^[ACGT]CC
PflMI         100  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          147  GAGTC                GAGTC(4/5)
SfaNI         158  GATGC                GCATC(5/9)
Van91I        100  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwNI          67  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              135  CAGCTGCTG           
AspHI         189  GAGCTC               G[AT]GC[AT]^C
              231  GAGCTC              
BsiHKAI       189  GAGCTC               G[AT]GC[AT]^C
              231  GAGCTC              
BsiYI         100  CCAAGCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              200  CCTGTGACAGG         
BslI          100  CCAAGCTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              200  CCTGTGACAGG         
Cfr13I        118  GGGCC                G^G[ACGT]CC
              119  GGCCC               
FauI          227  GCGGG                CCCGC(4/6)
              256  GCGGG               
HaeIII        119  GGCC                 GG^CC
              244  GGCC                
HphI          150  TCACC                GGTGA(8/7)
              171  TCACC               
MaeIII        149  GTCAC                ^GT[ACGT]AC
              203  GTGAC               
SacI          189  GAGCTC               GAGCT^C
              231  GAGCTC              
Sau96I        118  GGGCC                G^G[ACGT]CC
              119  GGCCC               
Tsp45I        149  GTCAC                ^GT[GC]AC
              203  GTGAC               
TspRI          25  CAGTG                CA[GC]TG[ACGT][ACGT]^
               67  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BpmI        Bsp1286I    Cac8I       Fnu4HI      ItaI        
MnlI        MspA1I      MwoI        PvuII       TseI