##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanII       BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HapII       HincII      HindII      HindIII     HpaI        
HpaII       KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        NaeI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AcyI           15  GGCGCC               G[GA]^CG[CT]C
AfaI           46  GTAC                 GT^AC
AluI          134  AGCT                 AG^CT
AlwI          199  GGATC                GGATC(4/5)
BbeI           15  GGCGCC               GGCGC^C
BbvCI         130  CCTCAGC              CCTCAGC(-5/-2)
BsaHI          15  GGCGCC               G[GA]^CG[CT]C
BsmAI          75  GAGAC                GTCTC(1/5)
BsrBI         104  CCGCTC               CCGCTC(-3/-3)
BstF5I          3  GGATG                GGATG(2/0)
DpnII         200  GATC                 ^GATC
DraII          32  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI          121  TGGCCG               [CT]^GGCC[GA]
EarI           59  CTCTTC               CTCTTC(1/4)
EcoO109I       32  GGGTCCC              [GA]G^G[ACGT]CC[CT]
FokI            3  GGATG                GGATG(9/13)
Hin1I          15  GGCGCC               G[GA]^CG[CT]C
HinfI         231  GAATC                G^A[ACGT]TC
KasI           15  GGCGCC               G^GCGCC
Ksp632I        59  CTCTTC               CTCTTC(1/4)
MboI          200  GATC                 ^GATC
NarI           15  GGCGCC               GG^CGCC
NdeII         200  GATC                 ^GATC
PpuMI          32  GGGTCCC              [GA]G^G[AT]CC[CT]
RsaI           46  GTAC                 GT^AC
SapI           58  GCTCTTC              GCTCTTC(1/4)
Sau3AI        200  GATC                 ^GATC
SfoI           15  GGCGCC               GGC^GCC
TfiI          231  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaII          33  GGTCC                G^G[AT]CC
               88  GGACC               
BanI           15  GGCGCC               G^G[CT][GA]CC
              156  GGCACC              
BsaJI         188  CCCAGG               C^C[ACGT][ACGT]GG
              189  CCAGGG              
BsmFI          34  GTCCC                GGGAC(10/14)
              168  GTCCC               
BssKI          91  CCTGG                ^CC[ACGT]GG
              189  CCAGG               
BstNI          91  CCTGG                CC^[AT]GG
              189  CCAGG               
Cfr13I         33  GGTCC                G^G[ACGT]CC
               88  GGACC               
DdeI           73  CTGAG                C^T[ACGT]AG
              131  CTCAG               
EcoRII         91  CCTGG                ^CC[AT]GG
              189  CCAGG               
HaeII          15  GGCGCC               [GA]GCGC^[CT]
               94  GGCGCT              
HaeIII        122  GGCC                 GG^CC
              176  GGCC                
HgaI           10  GACGC                GACGC(5/10)
              147  GCGTC               
HphI          240  GGTGA                GGTGA(8/7)
              180  TCACC               
MboII          60  TCTTC                GAAGA(8/7)
              111  TCTTC               
MvaI           91  CCTGG                CC^[AT]GG
              189  CCAGG               
Sau96I         33  GGTCC                G^G[ACGT]CC
               88  GGACC               
ScrFI          91  CCTGG                CC^[ACGT]GG
              189  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AccII       AciI        BbvI        BsiYI       BslI        
BstUI       Cac8I       CfoI        FauI        Fnu4HI      
HhaI        HinP1I      ItaI        MnlI        MspA1I      
MvnI        MwoI        NlaIV       TseI