Restriction map for
pF1KE0244
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI Fnu4HI FseI FspI HaeII
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI TfiI TseI Tth111I
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 196 GAATTT [GA]^AATT[CT]
AluI 370 AGCT AG^CT
ApoI 196 GAATTT [GA]^AATT[CT]
BanII 270 GGGCTC G[GA]GC[CT]^C
BmyI 270 GGGCTC G[AGT]GC[ACT]^C
BsaAI 58 CACGTA [CT]AC^GT[GA]
BsaJI 341 CCTTGG C^C[ACGT][ACGT]GG
BsiYI 57 CCACGTATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 57 CCACGTATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 270 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 291 TGTACA T^GTACA
BspMI 52 ACCTGC ACCTGC(4/8)
BsrGI 291 TGTACA T^GTACA
BsrI 84 ACTGG ACTGG(1/-1)
BssKI 388 CCAGG ^CC[ACGT]GG
BstBI 23 TTCGAA TT^CGAA
BstF5I 111 CATCC GGATG(2/0)
BstNI 388 CCAGG CC^[AT]GG
CfoI 186 GCGC GCG^C
EcoRII 388 CCAGG ^CC[AT]GG
EcoT14I 341 CCTTGG C^C[AT][AT]GG
FokI 111 CATCC GGATG(9/13)
HhaI 186 GCGC GCG^C
HinP1I 186 GCGC G^CGC
HphI 41 TCACC GGTGA(8/7)
MaeIII 40 GTCAC ^GT[ACGT]AC
MvaI 388 CCAGG CC^[AT]GG
MwoI 177 GCTACCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 282 CATG CATG^
NspV 23 TTCGAA TT^CGAA
PshAI 144 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScrFI 388 CCAGG CC^[ACGT]GG
SfuI 23 TTCGAA TT^CGAA
SmlI 265 CTTGAG C^T[CT][GA]AG
SspBI 291 TGTACA T^GTACA
SspI 208 AATATT AAT^ATT
StyI 341 CCTTGG C^C[AT][AT]GG
Tsp45I 40 GTCAC ^GT[GC]AC
Tsp509I 197 AATT ^AATT
TspRI 360 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 397 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 292 GTAC GT^AC
320 GTAC
AvaII 143 GGACC G^G[AT]CC
385 GGTCC
BclI 93 TGATCA T^GATCA
356 TGATCA
BfaI 314 CTAG C^TAG
398 CTAG
BsmFI 76 GGGAC GGGAC(10/14)
142 GGGAC
Cac8I 226 GCCTGC GC[ACGT]^[ACGT]GC
297 GCCTGC
Cfr13I 143 GGACC G^G[ACGT]CC
385 GGTCC
DpnII 94 GATC ^GATC
357 GATC
FbaI 93 TGATCA T^GATCA
356 TGATCA
HinfI 78 GACTC G^A[ACGT]TC
309 GACTC
MaeI 314 CTAG C^TAG
398 CTAG
MboI 94 GATC ^GATC
357 GATC
MseI 32 TTAA T^TAA
212 TTAA
NdeII 94 GATC ^GATC
357 GATC
NlaIV 142 GGGACC GG[ACGT]^[ACGT]CC
271 GGCTCC
PleI 78 GACTC GAGTC(4/5)
309 GACTC
RsaI 292 GTAC GT^AC
320 GTAC
Sau3AI 94 GATC ^GATC
357 GATC
Sau96I 143 GGACC G^G[ACGT]CC
385 GGTCC
TaqI 24 TCGA T^CGA
81 TCGA
Tru9I 32 TTAA T^TAA
212 TTAA
TthHB8I 24 TCGA T^CGA
81 TCGA
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
MaeII MboII MnlI