Restriction map for
pF1KE0241
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbvCI BciVI BclI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiWI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HpaI
HpaII KasI KspI MamI MflI
MluI MroI MseI MspA1I MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PstI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 200 GGTACC G^GTACC
AcsI 1113 AAATTC [GA]^AATT[CT]
AflIII 223 ACGTGT A^C[GA][CT]GT
AlwI 735 GGATC GGATC(4/5)
ApoI 1113 AAATTC [GA]^AATT[CT]
Asp700I 704 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 200 GGTACC G^GTACC
AvaII 333 GGACC G^G[AT]CC
BanI 200 GGTACC G^G[CT][GA]CC
BanII 1249 GAGCCC G[GA]GC[CT]^C
BbrPI 222 CACGTG CAC^GTG
BcgI 118 CGACAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 222 CACGTG [CT]AC^GT[GA]
BsaI 412 GAGACC GGTCTC(1/5)
BseRI 691 CTCCTC GAGGAG(10/8)
BsgI 126 GTGCAG GTGCAG(16/14)
BsiEI 774 CGATCG CG[GA][CT]^CG
BsiYI 660 CCACTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 660 CCACTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 457 GAATGC GAATGC(1/-1)
BstXI 221 CCACGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 530 GCGC GCG^C
DdeI 1080 CTTAG C^T[ACGT]AG
EcoT22I 341 ATGCAT ATGCA^T
HhaI 530 GCGC GCG^C
HinP1I 530 GCGC G^CGC
HincII 376 GTCAAC GT[CT]^[GA]AC
HindII 376 GTCAAC GT[CT]^[GA]AC
HindIII 359 AAGCTT A^AGCTT
KpnI 200 GGTACC GGTAC^C
MaeII 223 ACGT A^CGT
MfeI 464 CAATTG C^AATTG
MunI 464 CAATTG C^AATTG
NsiI 341 ATGCAT ATGCA^T
PflMI 660 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 222 CACGTG CAC^GTG
PmlI 222 CACGTG CAC^GTG
PpuMI 332 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 573 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 774 CGATCG CGAT^CG
SpeI 1192 ACTAGT A^CTAGT
TfiI 770 GAATC G^A[AT]TC
Van91I 660 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1245 TCTAGA T^CTAGA
XmnI 704 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 683 GAGCAC G[AT]GC[AT]^C
985 GTGCTC
BbvI 391 GCTGC GCAGC(8/12)
503 GCTGC
BsiHKAI 683 GAGCAC G[AT]GC[AT]^C
985 GTGCTC
BsmBI 33 CGTCTC CGTCTC(1/5)
611 GAGACG
BspDI 511 ATCGAT AT^CGAT
772 ATCGAT
BsrI 891 ACTGG ACTGG(1/-1)
489 CCAGT
Cac8I 239 GCTGGC GC[ACGT]^[ACGT]GC
617 GCAAGC
ClaI 511 ATCGAT AT^CGAT
772 ATCGAT
DpnII 736 GATC ^GATC
775 GATC
DraII 332 GGGACCT [GA]G^G[ACGT]CC[CT]
1255 AGGCCCT
DraIII 219 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
661 CACTTGGTG
DsaI 68 CCATGG C^C[GA][CT]GG
327 CCATGG
EcoO109I 332 GGGACCT [GA]G^G[ACGT]CC[CT]
1255 AGGCCCT
EcoT14I 68 CCATGG C^C[AT][AT]GG
327 CCATGG
HphI 184 GGTGA GGTGA(8/7)
218 TCACC
MaeIII 448 GTCAC ^GT[ACGT]AC
587 GTCAC
MboI 736 GATC ^GATC
775 GATC
NcoI 68 CCATGG C^CATGG
327 CCATGG
NdeII 736 GATC ^GATC
775 GATC
PleI 573 GACTC GAGTC(4/5)
1270 GAGTC
Sau3AI 736 GATC ^GATC
775 GATC
SfcI 24 CTACAG C^T[GA][CT]AG
803 CTATAG
StyI 68 CCATGG C^C[AT][AT]GG
327 CCATGG
TseI 391 GCTGC G^C[AT]GC
503 GCTGC
Tsp45I 448 GTCAC ^GT[GC]AC
587 GTCAC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI BalI BbsI
BfaI BglI BmyI BpuAI BsaJI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cfr13I EaeI EarI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MboII MluNI MnlI
MscI MslI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TaqI Tsp509I TspRI TthHB8I