Restriction map for
pF1KE0236
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AclI 390 AACGTT AA^CGTT
AcsI 3 GAATTC [GA]^AATT[CT]
AcyI 346 GACGCC G[GA]^CG[CT]C
AfaI 270 GTAC GT^AC
AocI 273 CCTGAGG CC^T[ACGT]AGG
ApaI 561 GGGCCC GGGCC^C
ApoI 3 GAATTC [GA]^AATT[CT]
AvaII 192 GGTCC G^G[AT]CC
BanI 412 GGCACC G^G[CT][GA]CC
BbsI 529 GTCTTC GAAGAC(2/6)
BbvCI 638 CCTCAGC CCTCAGC(-5/-2)
BfaI 116 CTAG C^TAG
BpuAI 529 GTCTTC GAAGAC(2/6)
BsaHI 346 GACGCC G[GA]^CG[CT]C
BsaI 553 GAGACC GGTCTC(1/5)
BsmAI 553 GAGAC GTCTC(1/5)
BsmFI 175 GGGAC GGGAC(10/14)
BsmI 14 GAATGC GAATGC(1/-1)
BspMI 254 GCAGGT ACCTGC(4/8)
BstBI 386 TTCGAA TT^CGAA
Bsu36I 273 CCTGAGG CC^T[ACGT]AGG
DpnII 327 GATC ^GATC
DraI 403 TTTAAA TTT^AAA
DraII 191 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 184 GACAGGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 236 TGGCCG [CT]^GGCC[GA]
Eco81I 273 CCTGAGG CC^T[ACGT]AGG
EcoO109I 191 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3 GAATTC G^AATTC
FauI 189 GCGGG CCCGC(4/6)
HgaI 346 GACGC GACGC(5/10)
Hin1I 346 GACGCC G[GA]^CG[CT]C
MaeI 116 CTAG C^TAG
MaeII 391 ACGT A^CGT
MaeIII 479 GTCAC ^GT[ACGT]AC
MboI 327 GATC ^GATC
MslI 336 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 238 GCCGTCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 327 GATC ^GATC
NspI 447 GCATGT [GA]CATG^[CT]
NspV 386 TTCGAA TT^CGAA
PleI 770 GACTC GAGTC(4/5)
PpuMI 191 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 390 AACGTT AA^CGTT
RsaI 270 GTAC GT^AC
SapI 453 GAAGAGC GCTCTTC(1/4)
Sau3AI 327 GATC ^GATC
SexAI 524 ACCAGGT A^CC[AT]GGT
SfaNI 650 GCATC GCATC(5/9)
SfuI 386 TTCGAA TT^CGAA
TfiI 72 GAATC G^A[AT]TC
Tsp45I 479 GTCAC ^GT[GC]AC
Tsp509I 4 AATT ^AATT
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# TWO CUT ENZYMES ( 13 enzymes) #
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Asp700I 85 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
537 GAATGAGTTC
BanII 373 GAGCCC G[GA]GC[CT]^C
561 GGGCCC
BseRI 265 GAGGAG GAGGAG(10/8)
627 GAGGAG
BspHI 308 TCATGA T^CATGA
753 TCATGA
Cac8I 153 GCTCGC GC[ACGT]^[ACGT]GC
646 GCTTGC
HinfI 72 GAATC G^A[ACGT]TC
770 GACTC
HphI 463 GGTGA GGTGA(8/7)
75 TCACC
MseI 404 TTAA T^TAA
797 TTAA
RcaI 308 TCATGA T^CATGA
753 TCATGA
SspI 712 AATATT AAT^ATT
803 AATATT
Tru9I 404 TTAA T^TAA
797 TTAA
TspRI 44 CACTG CA[GC]TG[ACGT][ACGT]^
225 CACTG
XmnI 85 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
537 GAATGAGTTC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI EarI
EcoRII Fnu4HI FokI HaeIII ItaI
Ksp632I MboII MnlI MvaI NlaIII
NlaIV Sau96I ScrFI TaqI TseI
TthHB8I