##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HinfI       HpaI        HphI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MunI        
MvaI        MvnI        NciI        NcoI        NdeI        
NdeII       NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfiI        
SfuI        SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AcyI          248  GGCGCC               G[GA]^CG[CT]C
AfaI           59  GTAC                 GT^AC
BbeI          248  GGCGCC               GGCGC^C
BsaHI         248  GGCGCC               G[GA]^CG[CT]C
BsmBI         235  CGTCTC               CGTCTC(1/5)
BsrI          102  CCAGT                ACTGG(1/-1)
BstXI         309  CCACAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          249  GCGC                 GCG^C
DraII         229  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      229  AGGCCCC              [GA]G^G[ACGT]CC[CT]
HaeII         248  GGCGCC               [GA]GCGC^[CT]
HaeIII        230  GGCC                 GG^CC
HhaI          249  GCGC                 GCG^C
Hin1I         248  GGCGCC               G[GA]^CG[CT]C
HinP1I        249  GCGC                 G^CGC
KasI          248  GGCGCC               G^GCGCC
MslI           74  CACCTAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          251  GCCGGC               GCC^GGC
NarI          248  GGCGCC               GG^CGCC
NgoMIV        251  GCCGGC               G^CCGGC
NlaIII        116  CATG                 CATG^
PstI          125  CTGCAG               CTGCA^G
RsaI           59  GTAC                 GT^AC
SfcI          125  CTGCAG               C^T[GA][CT]AG
SfoI          248  GGCGCC               GGC^GCC
SgrAI         244  CACCGGCG             C[GA]^CCGG[CT]G


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaII         145  GGTCC                G^G[AT]CC
              169  GGTCC               
BanI          248  GGCGCC               G^G[CT][GA]CC
              254  GGCACC              
BanII         261  GAGCCC               G[GA]GC[CT]^C
              294  GGGCTC              
BsmAI         130  GTCTC                GTCTC(1/5)
              236  GTCTC               
BsrFI         245  ACCGGC               [GA]^CCGG[CT]
              251  GCCGGC              
Cfr10I        245  ACCGGC               [GA]^CCGG[CT]
              251  GCCGGC              
DdeI           77  CTAAG                C^T[ACGT]AG
              217  CTGAG               
EarI          132  CTCTTC               CTCTTC(1/4)
              238  CTCTTC              
HapII         246  CCGG                 C^CGG
              252  CCGG                
HpaII         246  CCGG                 C^CGG
              252  CCGG                
Ksp632I       132  CTCTTC               CTCTTC(1/4)
              238  CTCTTC              
MboII         133  TCTTC                GAAGA(8/7)
              239  TCTTC               
MnlI           37  CCTC                 CCTC(7/6)
               49  CCTC                
MspI          246  CCGG                 C^CGG
              252  CCGG                
TspRI          25  CAGTG                CA[GC]TG[ACGT][ACGT]^
              103  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BbvI        BmyI        BsiYI       BslI        
BsmFI       Bsp1286I    Cac8I       Cfr13I      Fnu4HI      
ItaI        MwoI        NlaIV       Sau96I      TseI