##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TfiI        TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AfaI          311  GTAC                 GT^AC
AlwI           41  GATCC                GGATC(4/5)
Asp700I       670  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         535  GGTCC                G^G[AT]CC
BbvI          417  GCAGC                GCAGC(8/12)
BclI          444  TGATCA               T^GATCA
BfaI          130  CTAG                 C^TAG
BglII         303  AGATCT               A^GATCT
BsaJI          44  CCCCGG               C^C[ACGT][ACGT]GG
BsaWI         540  ACCGGA               [AT]^CCGG[AT]
BspMI         613  GCAGGT               ACCTGC(4/8)
BstBI         197  TTCGAA               TT^CGAA
Cac8I         168  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          463  GCGC                 GCG^C
FbaI          444  TGATCA               T^GATCA
Fnu4HI        417  GCAGC                GC^[ACGT]GC
HaeII         462  AGCGCC               [GA]GCGC^[CT]
HhaI          463  GCGC                 GCG^C
HinP1I        463  GCGC                 G^CGC
HinfI         469  GACTC                G^A[ACGT]TC
HphI           54  GGTGA                GGTGA(8/7)
ItaI          417  GCAGC                GC^[ACGT]GC
MaeI          130  CTAG                 C^TAG
MaeIII         55  GTGAC                ^GT[ACGT]AC
MwoI          168  GCTGGCCCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV          197  TTCGAA               TT^CGAA
PleI          469  GACTC                GAGTC(4/5)
PstI          415  CTGCAG               CTGCA^G
RsaI          311  GTAC                 GT^AC
SfaNI         772  GATGC                GCATC(5/9)
SfcI          415  CTGCAG               C^T[GA][CT]AG
SfuI          197  TTCGAA               TT^CGAA
StuI          105  AGGCCT               AGG^CCT
TseI          417  GCAGC                G^C[AT]GC
Tsp45I         55  GTGAC                ^GT[GC]AC
XmnI          670  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbsI          778  GAAGAC               GAAGAC(2/6)
              746  GTCTTC              
BcnI           45  CCCGG                CC^[GC]GG
              173  CCCGG               
BpmI           25  CTGGAG               CTGGAG(16/14)
              230  CTGGAG              
BpuAI         778  GAAGAC               GAAGAC(2/6)
              746  GTCTTC              
BsiYI          45  CCCGGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              538  CCACCGGAAGG         
BslI           45  CCCGGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              538  CCACCGGAAGG         
BstYI          40  AGATCC               [GA]^GATC[CT]
              303  AGATCT              
MflI           40  AGATCC               [GA]^GATC[CT]
              303  AGATCT              
NciI           45  CCCGG                CC^[GC]GG
              173  CCCGG               
TaqI          198  TCGA                 T^CGA
              750  TCGA                
TthHB8I       198  TCGA                 T^CGA
              750  TCGA                
XhoII          40  AGATCC               [GA]^GATC[CT]
              303  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AcsI        AluI        ApoI        BseRI       BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EarI        EcoRII      FokI        HaeIII      HapII       
HpaII       Ksp632I     MboI        MboII       MnlI        
MseI        MspI        MvaI        NdeII       Sau3AI      
Sau96I      ScrFI       Tru9I       Tsp509I