Restriction map for
pF1KE0180
##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FokI FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tsp509I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AlwI 240 GGATC GGATC(4/5)
AlwNI 139 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 317 GTGCTC G[AT]GC[AT]^C
AspI 90 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 634 CCCGAG C^[CT]CG[GA]G
BanI 508 GGCACC G^G[CT][GA]CC
BbsI 421 GTCTTC GAAGAC(2/6)
BlpI 54 GCTGAGC GC^T[ACGT]AGC
BpmI 145 CTGGAG CTGGAG(16/14)
Bpu1102I 54 GCTGAGC GC^T[ACGT]AGC
BpuAI 421 GTCTTC GAAGAC(2/6)
BsaI 251 GGTCTC GGTCTC(1/5)
BseRI 537 CTCCTC GAGGAG(10/8)
BsiHKAI 317 GTGCTC G[AT]GC[AT]^C
BsoBI 634 CCCGAG C^[CT]CG[GA]G
BspDI 487 ATCGAT AT^CGAT
BspMI 75 ACCTGC ACCTGC(4/8)
CelII 54 GCTGAGC GC^T[ACGT]AGC
CfoI 129 GCGC GCG^C
ClaI 487 ATCGAT AT^CGAT
DraII 65 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 176 CCACGG C^C[GA][CT]GG
EaeI 576 TGGCCG [CT]^GGCC[GA]
EcoO109I 65 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 490 GATATC GAT^ATC
EcoT14I 330 CCAAGG C^C[AT][AT]GG
HhaI 129 GCGC GCG^C
HinP1I 129 GCGC G^CGC
HincII 169 GTTAAC GT[CT]^[GA]AC
HindII 169 GTTAAC GT[CT]^[GA]AC
HpaI 169 GTTAAC GTT^AAC
HphI 401 TCACC GGTGA(8/7)
MaeII 263 ACGT A^CGT
MboII 422 TCTTC GAAGA(8/7)
MseI 170 TTAA T^TAA
MspA1I 346 CCGCTG C[AC]G^C[GT]G
PflFI 90 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 65 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 562 CTGCAG CTGCA^G
SfaNI 641 GCATC GCATC(5/9)
SfcI 562 CTGCAG C^T[GA][CT]AG
StyI 330 CCAAGG C^C[AT][AT]GG
TaqI 488 TCGA T^CGA
Tru9I 170 TTAA T^TAA
Tth111I 90 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 488 TCGA T^CGA
XcmI 338 CCACTGCCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BcnI 579 CCGGG CC^[GC]GG
596 CCGGG
BmyI 317 GTGCTC G[AGT]GC[ACT]^C
629 GTGCCC
BsmAI 252 GTCTC GTCTC(1/5)
535 GTCTC
BsmFI 67 GTCCC GGGAC(10/14)
96 GTCCC
Bsp1286I 317 GTGCTC G[AGT]GC[ACT]^C
629 GTGCCC
BsrI 19 ACTGG ACTGG(1/-1)
549 ACTGG
HapII 579 CCGG C^CGG
596 CCGG
HpaII 579 CCGG C^CGG
596 CCGG
MslI 546 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
642 CATCGCTATG
MspI 579 CCGG C^CGG
596 CCGG
NciI 579 CCGGG CC^[GC]GG
596 CCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsgI BsiYI BslI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeIII HinfI ItaI
MboI MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI TseI TspRI