Restriction map for
pF1KE0176
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII BalI BanII BbeI
BbvCI BcgI BciVI BclI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflII 1144 CTTAAG C^TTAAG
AocI 587 CCTCAGG CC^T[ACGT]AGG
AseI 574 ATTAAT AT^TAAT
AsnI 574 ATTAAT AT^TAAT
Asp700I 1798 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 360 CCTAGG C^CTAGG
BamHI 1263 GGATCC G^GATCC
BanI 1501 GGCACC G^G[CT][GA]CC
BbrPI 1940 CACGTG CAC^GTG
BbsI 2326 GAAGAC GAAGAC(2/6)
BfrI 1144 CTTAAG C^TTAAG
BglI 2012 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 360 CCTAGG C^CTAGG
BlpI 1849 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1849 GCTCAGC GC^T[ACGT]AGC
BpuAI 2326 GAAGAC GAAGAC(2/6)
BsaAI 1940 CACGTG [CT]AC^GT[GA]
BsaI 1619 GGTCTC GGTCTC(1/5)
BsaWI 2193 ACCGGA [AT]^CCGG[AT]
BspLU11I 307 ACATGT A^CATGT
BspMI 1919 ACCTGC ACCTGC(4/8)
BsrDI 162 CATTGC GCAATG(2/0)
Bsu36I 587 CCTCAGG CC^T[ACGT]AGG
CelII 1849 GCTCAGC GC^T[ACGT]AGC
CfoI 2097 GCGC GCG^C
DraI 1717 TTTAAA TTT^AAA
Eco81I 587 CCTCAGG CC^T[ACGT]AGG
EcoRI 581 GAATTC G^AATTC
EcoT22I 159 ATGCAT ATGCA^T
FauI 1912 CCCGC CCCGC(4/6)
HhaI 2097 GCGC GCG^C
HinP1I 2097 GCGC G^CGC
HincII 2065 GTCGAC GT[CT]^[GA]AC
HindII 2065 GTCGAC GT[CT]^[GA]AC
MfeI 1573 CAATTG C^AATTG
MunI 1573 CAATTG C^AATTG
NsiI 159 ATGCAT ATGCA^T
PflMI 2291 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1940 CACGTG CAC^GTG
PmlI 1940 CACGTG CAC^GTG
PshBI 574 ATTAAT AT^TAAT
PvuII 1414 CAGCTG CAG^CTG
SalI 2065 GTCGAC G^TCGAC
SspI 1012 AATATT AAT^ATT
StuI 2114 AGGCCT AGG^CCT
Van91I 2291 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1338 TCTAGA T^CTAGA
XmnI 1798 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 307 ACATGT A^C[GA][CT]GT
1941 ACGTGT
AlwNI 373 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1294 CAGAGTCTG
AspHI 875 GTGCTC G[AT]GC[AT]^C
1680 GAGCAC
AvaI 1996 CCCGAG C^[CT]CG[GA]G
2184 CTCGGG
AvaII 35 GGTCC G^G[AT]CC
454 GGTCC
BcnI 710 CCGGG CC^[GC]GG
1484 CCCGG
BglII 801 AGATCT A^GATCT
809 AGATCT
BmyI 875 GTGCTC G[AGT]GC[ACT]^C
1680 GAGCAC
BsgI 1921 CTGCAC GTGCAG(16/14)
2241 GTGCAG
BsiHKAI 875 GTGCTC G[AT]GC[AT]^C
1680 GAGCAC
BsoBI 1996 CCCGAG C^[CT]CG[GA]G
2184 CTCGGG
Bsp1286I 875 GTGCTC G[AGT]GC[ACT]^C
1680 GAGCAC
Bst1107I 1165 GTATAC GTA^TAC
1691 GTATAC
BstZ17I 1165 GTATAC GTA^TAC
1691 GTATAC
EarI 1084 GAAGAG CTCTTC(1/4)
1217 GAAGAG
EcoNI 1200 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1268 CCTATTTTAGG
EcoRV 747 GATATC GAT^ATC
1328 GATATC
EcoT14I 360 CCTAGG C^C[AT][AT]GG
1807 CCAAGG
Ksp632I 1084 GAAGAG CTCTTC(1/4)
1217 GAAGAG
MslI 771 CATTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
845 CACTCTGGTG
NciI 710 CCGGG CC^[GC]GG
1484 CCCGG
NspI 307 ACATGT [GA]CATG^[CT]
1169 ACATGC
PleI 1296 GAGTC GAGTC(4/5)
1375 GAGTC
PpuMI 34 AGGTCCT [GA]G^G[AT]CC[CT]
453 AGGTCCC
PstI 910 CTGCAG CTGCA^G
1433 CTGCAG
StyI 360 CCTAGG C^C[AT][AT]GG
1807 CCAAGG
TaqI 1116 TCGA T^CGA
2066 TCGA
TthHB8I 1116 TCGA T^CGA
2066 TCGA
XcmI 819 CCATTCTTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
844 CCACTCTGGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI BbvI BfaI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII