Restriction map for
pF1KE0171
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 106 GGCGCC G[GA]^CG[CT]C
AfaI 204 GTAC GT^AC
AspHI 556 GAGCAC G[AT]GC[AT]^C
BalI 218 TGGCCA TGG^CCA
BamHI 501 GGATCC G^GATCC
BanII 60 GGGCTC G[GA]GC[CT]^C
BbeI 106 GGCGCC GGCGC^C
BbvCI 312 CCTCAGC CCTCAGC(-5/-2)
BcnI 9 CCGGG CC^[GC]GG
BpmI 63 CTCCAG CTGGAG(16/14)
BsaAI 172 TACGTG [CT]AC^GT[GA]
BsaHI 106 GGCGCC G[GA]^CG[CT]C
BsaWI 42 TCCGGT [AT]^CCGG[AT]
BseRI 196 GAGGAG GAGGAG(10/8)
BsiHKAI 556 GAGCAC G[AT]GC[AT]^C
BsmFI 468 GGGAC GGGAC(10/14)
BspMI 89 ACCTGC ACCTGC(4/8)
BsrI 626 ACTGG ACTGG(1/-1)
BstAPI 132 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 501 GGATCC [GA]^GATC[CT]
EaeI 218 TGGCCA [CT]^GGCC[GA]
EarI 248 CTCTTC CTCTTC(1/4)
HaeII 106 GGCGCC [GA]GCGC^[CT]
Hin1I 106 GGCGCC G[GA]^CG[CT]C
HphI 191 TCACC GGTGA(8/7)
KasI 106 GGCGCC G^GCGCC
Ksp632I 248 CTCTTC CTCTTC(1/4)
MboII 249 TCTTC GAAGA(8/7)
MflI 501 GGATCC [GA]^GATC[CT]
MluNI 218 TGGCCA TGG^CCA
MscI 218 TGGCCA TGG^CCA
MseI 392 TTAA T^TAA
NarI 106 GGCGCC GG^CGCC
NciI 9 CCGGG CC^[GC]GG
RsaI 204 GTAC GT^AC
SapI 247 GCTCTTC GCTCTTC(1/4)
SfaNI 412 GCATC GCATC(5/9)
SfoI 106 GGCGCC GGC^GCC
Tru9I 392 TTAA T^TAA
Tsp509I 480 AATT ^AATT
XcmI 26 CCAGCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 501 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 129 CGCG CG^CG
165 CGCG
AciI 20 GCGG CCGC(-3/-1)
166 GCGG
AlwI 502 GATCC GGATC(4/5)
501 GGATC
BanI 22 GGCACC G^G[CT][GA]CC
106 GGCGCC
BmyI 60 GGGCTC G[AGT]GC[ACT]^C
556 GAGCAC
BsgI 145 CTGCAC GTGCAG(16/14)
257 CTGCAC
Bsp1286I 60 GGGCTC G[AGT]GC[ACT]^C
556 GAGCAC
BstUI 129 CGCG CG^CG
165 CGCG
CfoI 107 GCGC GCG^C
130 GCGC
DdeI 313 CTCAG C^T[ACGT]AG
432 CTCAG
DpnII 135 GATC ^GATC
502 GATC
EcoT14I 221 CCAAGG C^C[AT][AT]GG
426 CCAAGG
HapII 9 CCGG C^CGG
43 CCGG
HhaI 107 GCGC GCG^C
130 GCGC
HinP1I 107 GCGC G^CGC
130 GCGC
HpaII 9 CCGG C^CGG
43 CCGG
MboI 135 GATC ^GATC
502 GATC
MspA1I 34 CAGCTG C[AC]G^C[GT]G
142 CAGCTG
MspI 9 CCGG C^CGG
43 CCGG
MvnI 129 CGCG CG^CG
165 CGCG
NdeII 135 GATC ^GATC
502 GATC
NlaIII 138 CATG CATG^
651 CATG
PvuII 34 CAGCTG CAG^CTG
142 CAGCTG
Sau3AI 135 GATC ^GATC
502 GATC
StuI 522 AGGCCT AGG^CCT
589 AGGCCT
StyI 221 CCAAGG C^C[AT][AT]GG
426 CCAAGG
Tsp45I 190 GTCAC ^GT[GC]AC
368 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstNI Cac8I EcoRII Fnu4HI
HaeIII ItaI MaeII MaeIII MnlI
MvaI MwoI NlaIV ScrFI TseI
TspRI