Restriction map for
pF1KE0164
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MboI
MflI MluI MluNI MscI MseI
MvnI NaeI NarI NdeI NdeII
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 74 TCCGGA T^CCGGA
AlwNI 313 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 164 AGCGCT AGC^GCT
AvaII 228 GGACC G^G[AT]CC
BlpI 4 GCTGAGC GC^T[ACGT]AGC
BpmI 214 CTGGAG CTGGAG(16/14)
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BsaI 88 GGTCTC GGTCTC(1/5)
BseAI 74 TCCGGA T^CCGGA
BseRI 51 GAGGAG GAGGAG(10/8)
BsmAI 89 GTCTC GTCTC(1/5)
BspEI 74 TCCGGA T^CCGGA
BspMI 144 GCAGGT ACCTGC(4/8)
BstEII 201 GGTCACC G^GT[ACGT]ACC
BstPI 201 GGTCACC G^GT[ACGT]ACC
Cac8I 109 GCCAGC GC[ACGT]^[ACGT]GC
CelII 4 GCTGAGC GC^T[ACGT]AGC
DdeI 5 CTGAG C^T[ACGT]AG
DsaI 44 CCATGG C^C[GA][CT]GG
Eco47III 164 AGCGCT AGC^GCT
EcoO65I 201 GGTCACC G^GT[ACGT]ACC
EcoT14I 44 CCATGG C^C[AT][AT]GG
EcoT22I 250 ATGCAT ATGCA^T
MfeI 404 CAATTG C^AATTG
MroI 74 TCCGGA T^CCGGA
MslI 253 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 404 CAATTG C^AATTG
NcoI 44 CCATGG C^CATGG
NlaIV 393 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 250 ATGCAT ATGCA^T
NspI 353 ACATGC [GA]CATG^[CT]
PflMI 192 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 10 CTGCAG CTGCA^G
SfcI 10 CTGCAG C^T[GA][CT]AG
SmlI 185 CTCAAG C^T[CT][GA]AG
StuI 131 AGGCCT AGG^CCT
StyI 44 CCATGG C^C[AT][AT]GG
TaqI 161 TCGA T^CGA
TfiI 274 GATTC G^A[AT]TC
Tsp509I 405 AATT ^AATT
TthHB8I 161 TCGA T^CGA
Van91I 192 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 231 CCGC CCGC(-3/-1)
34 GCGG
Alw44I 148 GTGCAC G^TGCAC
243 GTGCAC
ApaLI 148 GTGCAC G^TGCAC
243 GTGCAC
AspHI 148 GTGCAC G[AT]GC[AT]^C
243 GTGCAC
BcnI 21 CCGGG CC^[GC]GG
239 CCCGG
BmyI 148 GTGCAC G[AGT]GC[ACT]^C
243 GTGCAC
BsaJI 44 CCATGG C^C[ACGT][ACGT]GG
191 CCCAGG
BsaWI 74 TCCGGA [AT]^CCGG[AT]
98 TCCGGT
BsgI 25 GTGCAG GTGCAG(16/14)
102 GTGCAG
BsiHKAI 148 GTGCAC G[AT]GC[AT]^C
243 GTGCAC
Bsp1286I 148 GTGCAC G[AGT]GC[ACT]^C
243 GTGCAC
BsrDI 253 CATTGC GCAATG(2/0)
257 GCAATG
BsrI 153 CCAGT ACTGG(1/-1)
206 CCAGT
BstXI 206 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
335 CCAAGTGTGTGG
CfoI 107 GCGC GCG^C
165 GCGC
Cfr13I 228 GGACC G^G[ACGT]CC
237 GGCCC
HaeII 106 AGCGCC [GA]GCGC^[CT]
164 AGCGCT
HaeIII 132 GGCC GG^CC
237 GGCC
HhaI 107 GCGC GCG^C
165 GCGC
HinP1I 107 GCGC G^CGC
165 GCGC
MaeIII 202 GTCAC ^GT[ACGT]AC
331 GTGAC
MboII 78 GAAGA GAAGA(8/7)
381 GAAGA
MspA1I 111 CAGCTG C[AC]G^C[GT]G
316 CAGCTG
NciI 21 CCGGG CC^[GC]GG
239 CCCGG
PleI 40 GAGTC GAGTC(4/5)
54 GAGTC
PvuII 111 CAGCTG CAG^CTG
316 CAGCTG
Sau96I 228 GGACC G^G[ACGT]CC
237 GGCCC
Tsp45I 202 GTCAC ^GT[GC]AC
331 GTGAC
TspRI 154 CAGTG CA[GC]TG[ACGT][ACGT]^
207 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BbvI BsiYI BslI BssKI
BstF5I BstNI EcoRII Fnu4HI FokI
HapII HinfI HpaII HphI ItaI
MnlI MspI MvaI MwoI NlaIII
ScrFI SfaNI TseI