##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        Tsp45I      Tth111I     Van91I      
XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AlwNI         162  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       226  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           62  CCCGGG               C^[CT]CG[GA]G
BbsI          307  GAAGAC               GAAGAC(2/6)
BfaI          190  CTAG                 C^TAG
BpuAI         307  GAAGAC               GAAGAC(2/6)
BsmFI         355  GGGAC                GGGAC(10/14)
BsoBI          62  CCCGGG               C^[CT]CG[GA]G
BsrI          320  CCAGT                ACTGG(1/-1)
BstNI         161  CCAGG                CC^[AT]GG
EcoRII        161  CCAGG                ^CC[AT]GG
HaeIII        175  GGCC                 GG^CC
HindIII       375  AAGCTT               A^AGCTT
HphI           74  TCACC                GGTGA(8/7)
MaeI          190  CTAG                 C^TAG
MslI          133  CATGTAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          161  CCAGG                CC^[AT]GG
MwoI          167  GCTGTAATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        133  CATG                 CATG^
NspI          132  GCATGT               [GA]CATG^[CT]
PleI          242  GAGTC                GAGTC(4/5)
PstI           52  CTGCAG               CTGCA^G
SfcI           52  CTGCAG               C^T[GA][CT]AG
SmaI           62  CCCGGG               CCC^GGG
SspI          382  AATATT               AAT^ATT
TaqI          257  TCGA                 T^CGA
TfiI          214  GATTC                G^A[AT]TC
Tsp509I       314  AATT                 ^AATT
TspRI         321  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       257  TCGA                 T^CGA
XbaI          189  TCTAGA               T^CTAGA
XmaCI          62  CCCGGG               C^CCGGG
XmaI           62  CCCGGG               C^CCGGG
XmnI          226  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BsaJI          62  CCCGGG               C^C[ACGT][ACGT]GG
              161  CCAGGG              
BsiYI          11  CCTTAGTCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              156  CCGAACCAGGG         
BslI           11  CCTTAGTCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              156  CCGAACCAGGG         
DdeI           12  CTTAG                C^T[ACGT]AG
              128  CTGAG               
HinfI         214  GATTC                G^A[ACGT]TC
              242  GAGTC               
MboII         223  GAAGA                GAAGA(8/7)
              307  GAAGA               
MnlI           72  CCTC                 CCTC(7/6)
               45  GAGG                
MseI          116  TTAA                 T^TAA
              380  TTAA                
Tru9I         116  TTAA                 T^TAA
              380  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BbvI        BcnI        BssKI       Fnu4HI      
HapII       HpaII       ItaI        MspI        NciI        
ScrFI       TseI