Restriction map for
pF1KE0144
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MflI
MluI MluNI MroI MscI MslI
MspA1I MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AspHI 382 GAGCTC G[AT]GC[AT]^C
AvaI 74 CCCGGG C^[CT]CG[GA]G
AvaII 149 GGTCC G^G[AT]CC
BclI 120 TGATCA T^GATCA
BglI 442 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 406 CTGCAC GTGCAG(16/14)
BsiHKAI 382 GAGCTC G[AT]GC[AT]^C
BsmFI 150 GTCCC GGGAC(10/14)
BsoBI 74 CCCGGG C^[CT]CG[GA]G
BstNI 447 CCTGG CC^[AT]GG
DdeI 25 CTGAG C^T[ACGT]AG
DpnII 121 GATC ^GATC
DraI 268 TTTAAA TTT^AAA
DraII 148 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 18 TGGCCG [CT]^GGCC[GA]
EcoO109I 148 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 447 CCTGG ^CC[AT]GG
EcoRV 370 GATATC GAT^ATC
EcoT14I 377 CCAAGG C^C[AT][AT]GG
FbaI 120 TGATCA T^GATCA
MaeIII 209 GTGAC ^GT[ACGT]AC
MboI 121 GATC ^GATC
MfeI 293 CAATTG C^AATTG
MseI 269 TTAA T^TAA
MunI 293 CAATTG C^AATTG
MvaI 447 CCTGG CC^[AT]GG
NdeII 121 GATC ^GATC
NspI 202 GCATGT [GA]CATG^[CT]
PleI 98 GACTC GAGTC(4/5)
PpuMI 148 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 382 GAGCTC GAGCT^C
Sau3AI 121 GATC ^GATC
SmaI 74 CCCGGG CCC^GGG
StyI 377 CCAAGG C^C[AT][AT]GG
TfiI 228 GATTC G^A[AT]TC
Tru9I 269 TTAA T^TAA
Tsp45I 209 GTGAC ^GT[GC]AC
XmaCI 74 CCCGGG C^CCGGG
XmaI 74 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbvI 48 GCTGC GCAGC(8/12)
51 GCTGC
BfaI 137 CTAG C^TAG
263 CTAG
BpmI 197 CTCCAG CTGGAG(16/14)
300 CTCCAG
BsrI 181 ACTGG ACTGG(1/-1)
186 ACTGG
BstF5I 237 GGATG GGATG(2/0)
258 GGATG
FokI 237 GGATG GGATG(9/13)
258 GGATG
HapII 75 CCGG C^CGG
91 CCGG
HinfI 98 GACTC G^A[ACGT]TC
228 GATTC
HpaII 75 CCGG C^CGG
91 CCGG
HphI 208 GGTGA GGTGA(8/7)
324 GGTGA
MaeI 137 CTAG C^TAG
263 CTAG
MaeII 143 ACGT A^CGT
212 ACGT
MspI 75 CCGG C^CGG
91 CCGG
NlaIII 203 CATG CATG^
417 CATG
PflMI 199 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
314 CCACTGAATGG
TseI 48 GCTGC G^C[AT]GC
51 GCTGC
TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^
409 CACTG
Van91I 199 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
314 CCACTGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BanII BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
Cac8I Cfr13I FauI Fnu4HI HaeIII
ItaI MboII MnlI MwoI NciI
NlaIV Sau96I ScrFI SfaNI Tsp509I