Restriction map for
pF1KA0408
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AseI AsnI Asp718I
AspEI AspI AvrII BbrPI BbvCI
BciVI BclI BfrI BlnI BsaBI
BsmI Bsp1407I BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
DraIII DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HpaI KpnI MamI MfeI MluI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I SalI SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI StyI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 700 TCCGGA T^CCGGA
AocI 1863 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2201 AGCGCT AGC^GCT
AscI 2121 GGCGCGCC GG^CGCGCC
Asp700I 2634 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1220 GGATCC G^GATCC
BglII 1569 AGATCT A^GATCT
BsaAI 2536 TACGTG [CT]AC^GT[GA]
BsaI 901 GAGACC GGTCTC(1/5)
BsaWI 700 TCCGGA [AT]^CCGG[AT]
BseAI 700 TCCGGA T^CCGGA
BsgI 79 CTGCAC GTGCAG(16/14)
BsiWI 600 CGTACG C^GTACG
BsmBI 1963 GAGACG CGTCTC(1/5)
BspDI 1108 ATCGAT AT^CGAT
BspEI 700 TCCGGA T^CCGGA
BspHI 1901 TCATGA T^CATGA
BspMI 1943 ACCTGC ACCTGC(4/8)
BstXI 1793 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1863 CCTTAGG CC^T[ACGT]AGG
ClaI 1108 ATCGAT AT^CGAT
CpoI 420 CGGACCG CG^G[AT]CCG
DraI 2803 TTTAAA TTT^AAA
EagI 818 CGGCCG C^GGCCG
EclXI 818 CGGCCG C^GGCCG
Eco47III 2201 AGCGCT AGC^GCT
Eco52I 818 CGGCCG C^GGCCG
Eco81I 1863 CCTTAGG CC^T[ACGT]AGG
HincII 1169 GTCAAC GT[CT]^[GA]AC
HindII 1169 GTCAAC GT[CT]^[GA]AC
HindIII 1451 AAGCTT A^AGCTT
MroI 700 TCCGGA T^CCGGA
NotI 817 GCGGCCGC GC^GGCCGC
PaeR7I 2674 CTCGAG C^TCGAG
PvuI 2430 CGATCG CGAT^CG
RcaI 1901 TCATGA T^CATGA
RsrII 420 CGGACCG CG^G[AT]CCG
SbfI 2100 CCTGCAGG CCTGCA^GG
SmaI 2404 CCCGGG CCC^GGG
SphI 989 GCATGC GCATG^C
Sse8387I 2100 CCTGCAGG CCTGCA^GG
SspI 2799 AATATT AAT^ATT
TfiI 1918 GAATC G^A[AT]TC
Tsp45I 120 GTCAC ^GT[GC]AC
XcmI 354 CCAGCCTGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2674 CTCGAG C^TCGAG
XmaCI 2404 CCCGGG C^CCGGG
XmaI 2404 CCCGGG C^CCGGG
XmnI 2634 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AviII 1301 TGCGCA TGC^GCA
2357 TGCGCA
BalI 1628 TGGCCA TGG^CCA
2540 TGGCCA
BbsI 1957 GAAGAC GAAGAC(2/6)
2812 GTCTTC
BglI 64 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
304 GCCGAGAAGGC
BlpI 607 GCTCAGC GC^T[ACGT]AGC
2142 GCTCAGC
Bpu1102I 607 GCTCAGC GC^T[ACGT]AGC
2142 GCTCAGC
BpuAI 1957 GAAGAC GAAGAC(2/6)
2812 GTCTTC
CelII 607 GCTCAGC GC^T[ACGT]AGC
2142 GCTCAGC
EarI 660 CTCTTC CTCTTC(1/4)
1499 GAAGAG
EcoNI 1308 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2100 CCTGCAGGAGG
FspI 1301 TGCGCA TGC^GCA
2357 TGCGCA
Ksp632I 660 CTCTTC CTCTTC(1/4)
1499 GAAGAG
MaeII 1985 ACGT A^CGT
2537 ACGT
MaeIII 120 GTCAC ^GT[ACGT]AC
245 GTAAC
MluNI 1628 TGGCCA TGG^CCA
2540 TGGCCA
MscI 1628 TGGCCA TGG^CCA
2540 TGGCCA
MslI 2532 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2718 CATTTCCGTG
NaeI 187 GCCGGC GCC^GGC
526 GCCGGC
NgoMIV 187 GCCGGC G^CCGGC
526 GCCGGC
NspI 989 GCATGC [GA]CATG^[CT]
2195 ACATGC
PpuMI 1316 AGGACCT [GA]G^G[AT]CC[CT]
2549 GGGACCT
StuI 1839 AGGCCT AGG^CCT
2636 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcgI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeI
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XhoII